FastQCFastQC Report
Tue 7 May 2024
EGAF00007822089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13883
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCG6474.6603759994237555No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCT4283.0829071526327163No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3522.535475041417561No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2451.764748253259382No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.7491176258733703No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG770.5546351653100915No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTC750.540229057120219No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAAT750.540229057120219No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC670.48260462436072893No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAG560.4033710293164301No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT540.3889649211265577No Hit
ATTGCGCAATGACAGGACTGGGGGCGCCATGCGCATCCTGAGCCGCATTG530.3817618670316214No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT500.36015270474681266No Hit
ATTGCGCAATGTAGTCGGGGGGCTATGGGCCCATCGACTACAAACGAAAC460.33134048836706764No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCG430.3097313260822589No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT430.3097313260822589No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC410.29532521789238636No Hit
ATTGCGCAATGTTAGTACAGGGAAGACAGGAAAAAGGAAAGCTTAAGGAA370.26651300151264135No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG340.2449038392278326No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT330.23770078513289636No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA330.23770078513289636No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG330.23770078513289636No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC310.22329467694302382No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGC300.21609162284808758No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC290.20888856875315134No Hit
ATTGCGCAATGAGATCCTAGGGGGTGGTCCAGCTATCGCTGGTTTCGCTA260.18727940646834257No Hit
ATTGCGCAATGTTCTATGGGGGTGTCGGTTGAGTCATTGTTGAAATACCA250.18007635237340633No Hit
ATTGCGCAATGACGACCGTGGGGGGGTCAAGTGAATAAGTGCATATGGTG240.1728732982784701No Hit
ATTGCGCAATGAAGGGTGTGGGGATGATGTGGTTTAATTCGATGCAACGC240.1728732982784701No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC240.1728732982784701No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGT220.15846719008859755No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAG220.15846719008859755No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT220.15846719008859755No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT220.15846719008859755No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG220.15846719008859755No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA210.15126413599366131No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG200.14406108189872505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT200.14406108189872505No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCGCG190.1368580278037888No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG180.12965497370885257No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT170.1224519196139163No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC160.11524886551898006No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCT160.11524886551898006No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGC160.11524886551898006No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGGTG150.10804581142404379No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT150.10804581142404379No Hit
ATTGCGCAATGAACAAGGGGGGGTGAAGGATATCACTGTTAAGTGAACAG140.10084275732910752No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT140.10084275732910752No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT140.10084275732910752No Hit
ATTGCGCAATGCTGAGCCGGGGGAGTGAAGGATATCACTGTTAAGTGAAC140.10084275732910752No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)