FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13284
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTCTATCG5193.9069557362240293No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4353.274616079494128No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTCTATCT3792.8530563083408613No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2872.1604938271604937No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1611.2119843420656429No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG920.6925624811803673No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCAC700.5269497139415839No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC690.5194218608852755No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTCTAT580.4366154772658838No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTC560.4215597711532671No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT550.4140319180969587No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT530.3989762119843421No Hit
ATTGCGCAATGTGTAAGCGGGGCGAGCAATTTGGTAAAGAGAATATGCTA510.3839205058717254No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT460.34628124059018367No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC360.27100271002710025No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTCTATCG360.27100271002710025No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG340.25594700391448355No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACT310.2333634447455586No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA280.21077988557663355No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT250.18819632640770853No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCA230.17314062029509183No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC230.17314062029509183No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT210.15808491418247517No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG200.15055706112616682No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACGGTTCTATCG200.15055706112616682No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG200.15055706112616682No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTT190.1430292080698585No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT190.1430292080698585No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG190.1430292080698585No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT180.13550135501355012No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG180.13550135501355012No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC180.13550135501355012No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTCTATCT180.13550135501355012No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC170.12797350195724178No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC170.12797350195724178No Hit
ATTGCGCAATGGCGGTCCGGGCGGAAAGAAAAGAAAGGGGGAAGAAGAAA160.12044564890093346No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC160.12044564890093346No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG160.12044564890093346No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTCTATCGCG160.12044564890093346No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC150.11291779584462511No Hit
ATTGCGCAATGACTTAATCGGGCAGGACGTCATAGAGGGTGAGAATCCCG140.10538994278831677No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)