FastQCFastQC Report
Tue 7 May 2024
EGAF00007822095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9572
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCG3643.802758044295863No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2662.7789385708315923No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCT2592.705808608441287No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1541.6088591725867112No Hit
ATTGCGCAATGTAATATCCGGGAGAAATCTCCAGTAGAATAAAAGGGTAA981.023819473464271No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG630.6581696615127455No Hit
ATTGCGCAATGTCTGCATCGGGCACGGCGAACGGTGACCTGGCCAACTAC540.5641454241537819No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.5432511491851233No Hit
ATTGCGCAATGATCGTTGAGGGGAACCTTTACTATAGCTTTGCACTGGAC460.48056832427914753No Hit
ATTGCGCAATGTGGGGCAGGGGCCATACACCTGGAATGGTAACAAGGGAA450.47012118679481824No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC420.4387797743418303No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT350.3656498119515253No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTG350.3656498119515253No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCAT340.355202674467196No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT330.3447555369828667No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT310.3238612620142081No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACC280.29251984956122024No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCG260.27162557459256165No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC210.21938988717091515No Hit
ATTGCGCAATGTACATAAGGGGCATGGGTCACTCAGTTGGGAGGAGGGTA200.20894274968658588No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC200.20894274968658588No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA200.20894274968658588No Hit
ATTGCGCAATGGTAGCACGGGGCAGAAACAACTATGTGTAGGAGAGCTGA200.20894274968658588No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG200.20894274968658588No Hit
ATTGCGCAATGACCGCCCGGGGCAGAAACAACTATGTGTAGGAGAGCTGA190.19849561220225656No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG190.19849561220225656No Hit
ATTGCGCAATGGCTAAAGGGGGAGCGAACAGCAAAAAGGTAAAACAAAAG190.19849561220225656No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCAGCG170.177601337233598No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCT160.1671541997492687No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC160.1671541997492687No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT160.1671541997492687No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG140.14625992478061012No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG140.14625992478061012No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC140.14625992478061012No Hit
ATTGCGCAATGACTTGACAGGGGAGGCGCCTGTATTCGCTCAGCAAGCCT130.13581278729628082No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG130.13581278729628082No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCT130.13581278729628082No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT120.12536564981195153No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC110.11491851232762224No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATGG110.11491851232762224No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATT110.11491851232762224No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT110.11491851232762224No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCAGCT100.10447137484329294No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC100.10447137484329294No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCGCG100.10447137484329294No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)