FastQCFastQC Report
Tue 7 May 2024
EGAF00007822100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13342
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3642.728226652675761No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2551.9112576825063707No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGTGTTGTGGG1361.0193374306700644No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTGTGTTGTGGG1050.7869884575026233No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGTGTTGTGGT720.5396492280017988No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.4572028181681907No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.4272223055014241No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG490.36726128016789084No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG480.3597661520011992No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG450.3372807675011243No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGTGTTGTGTT450.3372807675011243No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC440.3297856393344326No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTGTGTTGTGGT440.3297856393344326No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGTGTTGTGTG440.3297856393344326No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGTGTTGTGTA400.299805126667666No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA390.2923099985009744No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTT370.27731974216759103No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGTGGTGTGGG360.2698246140008994No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTGTGGTGTGGG360.2698246140008994No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGTGTT330.24733922950082443No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG320.23984410133413284No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT310.2323489731674412No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC300.22485384500074954No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA290.21735871683405789No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA290.21735871683405789No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTGTGTTGTGTG270.20236846050067456No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC260.1948733323339829No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.18737820416729126No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA240.1798830760005996No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC240.1798830760005996No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGGGTTGTGGG230.17238794783390796No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGTGTTGTG230.17238794783390796No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC230.17238794783390796No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTGTGTTGTG220.1648928196672163No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC210.15739769150052466No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA190.14240743516714136No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA190.14240743516714136No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA180.1349123070004497No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC180.1349123070004497No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGT170.12741717883375805No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAA170.12741717883375805No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCT170.12741717883375805No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC170.12741717883375805No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.12741717883375805No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTGTGTTGGGGG160.11992205066706642No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTGGGTTGTGGG160.11992205066706642No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA160.11992205066706642No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTGTGTT160.11992205066706642No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG160.11992205066706642No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA150.11242692250037477No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAT150.11242692250037477No Hit
TTTTTTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA140.1049317943336831No Hit
CATCTGACGCTGCCGACGACGCGTGTGTTGTGGGGGGGGGGGGGGGGGGG140.1049317943336831No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTGTGTTGTGTT140.1049317943336831No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCTTGTGGTGTGTG140.1049317943336831No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)