FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14202
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCG5393.7952401070271793No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCT3832.6968032671454725No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3442.4221940571750458No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1901.337839740881566No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.8519926770877342No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1030.7252499647936911No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC980.6900436558231235No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT930.654837346852556No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTAT810.5703422053231939No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGG790.5562596817349669No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGT760.5351358963526264No Hit
ATTGCGCAATGTACATTGGGGGCAGACAGCTCCTTCCTGTAGAGCTGCTA730.5140121109702859No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT570.4013519222644698No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC530.3731868750880158No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT500.35206308970567524No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT470.33093930432333474No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG460.3238980425292212No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC430.3027742571468807No Hit
ATTGCGCAATGTATAAGTAGGGGGGGTGACGGCGTACCTTTTGTATAATG380.2675679481763132No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA360.25348542458808615No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG340.23940290099985917No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG340.23940290099985917No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC330.23236163920574565No Hit
ATTGCGCAATGACTGATTAGGGGGTTGATATGATTGGTCGGCAGTGAGCG320.22532037741163213No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC320.22532037741163213No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG280.19715533023517817No Hit
ATTGCGCAATGTAATCGATGGGACCAAAGGCCGCAAGTTCTACGGGCCTG280.19715533023517817No Hit
ATTGCGCAATGTATCCATGGGGACTTAACAAACCACTTAAAACAAACAGA280.19715533023517817No Hit
ATTGCGCAATGCACACCCGGGGGGAAAAAAACAACACCGAGACACAAGAA270.19011406844106463No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGG270.19011406844106463No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT270.19011406844106463No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGGTATCG250.17603154485283762No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG240.16899028305872413No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT230.1619490212646106No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT220.15490775947049712No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCG220.15490775947049712No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG220.15490775947049712No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC220.15490775947049712No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGA210.1478664976763836No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC210.1478664976763836No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT190.1337839740881566No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT190.1337839740881566No Hit
ATTGCGCAATGTCCGCCCGGGGGTGGACAGCACTGATGGACGAGAAGGGC190.1337839740881566No Hit
ATTGCGCAATGACTGATTCGGGTTGTGGGTGAGTGAGTGATCCTGTACTG180.12674271229404308No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCG180.12674271229404308No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT170.11970145049992958No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT170.11970145049992958No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG170.11970145049992958No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCGCG160.11266018870581607No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA150.10561892691170256No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTG150.10561892691170256No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC150.10561892691170256No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)