FastQCFastQC Report
Tue 7 May 2024
EGAF00007822107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11295
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCG5164.568393094289509No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCT3663.240371845949535No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2912.5763612217795484No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1921.699867197875166No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG750.6640106241699867No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC750.6640106241699867No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAAT740.6551571491810536No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAG720.6374501992031872No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTC640.5666223992917221No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT570.5046480743691899No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.4869411243913236No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT420.37184594953519257No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT390.3452855245683931No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT340.3010181496237273No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC340.3010181496237273No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCG300.2656042496679947No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA270.2390438247011952No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC260.23019034971226204No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA250.22133687472332889No Hit
ATTGCGCAATGTAGAACTTGGGGGGACTCTCTGTTTGGTAACCCAAATAT250.22133687472332889No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG250.22133687472332889No Hit
ATTGCGCAATGTAGGTCATGGGGTGGAGGTCCGAACCCACTTCAGTTAAA240.21248339973439576No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG240.21248339973439576No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGT220.19477644975652944No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG220.19477644975652944No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC210.18592297476759628No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG200.17706949977866313No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC190.16821602478972997No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT180.1593625498007968No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAG170.15050907481186365No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCGCG170.15050907481186365No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAAGCG150.13280212483399734No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG150.13280212483399734No Hit
ATTGCGCAATGATCACTTGGGGGGACTCTCTGTTTGGTAACCCAAATATC140.12394864984506418No Hit
ATTGCGCAATGGTTGGTCTGGGGCTCAGCTGCAGCAGAGCCAACAAGCTA130.11509517485613102No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT130.11509517485613102No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG130.11509517485613102No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT130.11509517485613102No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT130.11509517485613102No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGC130.11509517485613102No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC130.11509517485613102No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG120.10624169986719788No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT120.10624169986719788No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)