FastQCFastQC Report
Tue 7 May 2024
EGAF00007822111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14410
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTCTATCG6384.427480916030534No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4733.2824427480916034No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTCTATCT4082.8313671061762666No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2912.0194309507286605No Hit
ATTGCGCAATGTTCTGCACGGGGCGAACCTGGGGAACTGAAACATCTAAG1591.1034004163775157No Hit
ATTGCGCAATGAGGGACTGGGGGGGCCCATAGCTCAGTTGGGCGCTGAGG1160.8049965301873698No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.6245662734212353No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG840.5829285218598196No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTCTAT680.4718945176960444No Hit
ATTGCGCAATGATATCAACGGGGCGAGCCTGTGCACGAGGACAACGAAAT630.43719639139486466No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTC580.4024982650936849No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCAC580.4024982650936849No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC550.38167938931297707No Hit
ATTGCGCAATGAAGAAACGCGGGAGGCCAGGTGTGGTGGCTCACGCCTGT500.3469812630117973No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT500.3469812630117973No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT500.3469812630117973No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT460.31922276197085353No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA380.263705759888966No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG370.25676613462873005No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG350.24288688410825815No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC330.22900763358778628No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTCTATCG300.2081887578070784No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG260.18043025676613464No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT230.15961138098542677No Hit
ATTGCGCAATGTTCCTGCTGGGGTGTAAGAGCGCATGGTGGATGCCTTGG230.15961138098542677No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC220.15267175572519084No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC220.15267175572519084No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACGGTTCTATCG220.15267175572519084No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTT210.1457321304649549No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC210.1457321304649549No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC210.1457321304649549No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACT200.13879250520471895No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT190.131852879944483No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCA180.12491325468424704No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG180.12491325468424704No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC180.12491325468424704No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC170.1179736294240111No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGGTCTATCG170.1179736294240111No Hit
ATTGCGCAATGGCGAGAGGGGGGCGAATGGGGGGTGCAGGATAATCCTCT170.1179736294240111No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG170.1179736294240111No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTCTATCT160.11103400416377517No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCAC150.1040943789035392No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT150.1040943789035392No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG150.1040943789035392No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT150.1040943789035392No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)