FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13403
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTA9076.767141684697456No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT6454.8123554428113104No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3772.8128031037827355No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTCGCC1651.2310676714168471Illumina Single End PCR Primer 1 (96% over 25bp)
CTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGG1571.171379541893606No Hit
CTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTC1030.7684846676117287No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTG760.5670372304707901No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGA730.5446541818995747No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.4775050361859285No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.4700440199955234No Hit
ATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGG520.38797284190106696No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTA460.3432067447586361No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG440.32828471237782586No Hit
GTGTGTTCTTAATTGTGTTAATTAGGAATCTTTTATTTGTACCAGCAAGA420.3133626799970156No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC420.3133626799970156No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGGCGCC410.30590166380661044No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG400.2984406476162053No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGA380.28351861523539507No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA360.2685965828545848No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA350.26113556666417964No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG330.2462135342833694No Hit
GGAATCTTTTATTTGTACCAGCAAGAGGACACTTCATCCAGCCCATCGGC330.2462135342833694No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC330.2462135342833694No Hit
GAACTTACCCGACAAGGAATTTCGCTACCTTAGGATGGTTATAGTTACCA310.23129150190255915No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTA300.223830485712154No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC300.223830485712154No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTAC290.21636946952174885No Hit
CCTTCATCCTGCTCATGCCTAGATCACTTGGTTTCGGGTCTGATCCGTCT280.20890845333134375No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA250.18652540476012833No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG240.17906438856972318No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA240.17906438856972318No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC240.17906438856972318No Hit
ACTCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTT220.16414235618891293No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTCGGC210.1566813399985078Illumina Single End PCR Primer 1 (95% over 23bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC210.1566813399985078No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA200.14922032380810266No Hit
ATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAG190.14175930761769753No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGT190.14175930761769753No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGGGGTCGCC190.14175930761769753No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG190.14175930761769753No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT180.1342982914272924No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATCTCGGTGGTGGCC180.1342982914272924Illumina Single End PCR Primer 1 (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA170.1268372752368873No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA170.1268372752368873No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA160.11937625904648212No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC160.11937625904648212No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.11937625904648212No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA150.111915242856077No Hit
GTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGATC150.111915242856077No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAGTACGAG140.10445422666567188No Hit
CATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGTTCTCGGTGGTCGCC140.10445422666567188No Hit
CTTATACACATCTGACGCTGCCGACGAAAGTACGAGAGTGTAGTTCTCGG140.10445422666567188No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)