FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9395
Sequences flagged as poor quality0
Sequence length151
%GC59

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCG4574.8642895156998405No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3423.6402341671101652No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCT3373.587014369345397No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1882.001064395955295No Hit
ATTGCGCAATGGACGATTGGGGGCTCCGGCGGAACGCTGGAGAGATGGCC670.7131452900478977No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCG550.5854177754124534No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGAT530.564129856306546No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.5428419372006386No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG460.48962213943587013No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTA440.46833422032996275No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC430.45769026077700903No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT390.4151144225651942No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT340.3618946248004257No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT270.28738690792974986No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA250.2660989888238425No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG250.2660989888238425No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC210.22352315061202765No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCT200.21287919105907396No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGAGCG200.21287919105907396No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG190.20223523150612027No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGC180.19159127195316658No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAT170.18094731240021286No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT170.18094731240021286No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC170.18094731240021286No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG170.18094731240021286No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCGCG170.18094731240021286No Hit
ATTGCGCAATGGGGACGGGGGGGGGCTCAATGTGGTGAAAACGGGCCGAG160.17030335284725917No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCG160.17030335284725917No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC150.15965939329430548No Hit
ATTGCGCAATGTTGCAGGCGGGGTGTACTGTCTTGCCGGGCCTTTCCTCT150.15965939329430548No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG140.1490154337413518No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT130.13837147418839807No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130.13837147418839807No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC130.13837147418839807No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC130.13837147418839807No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC120.12772751463544438No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT120.12772751463544438No Hit
GGGGGGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120.12772751463544438No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC110.11708355508249069No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT110.11708355508249069No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT110.11708355508249069No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT100.10643959552953698No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTG100.10643959552953698No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCT100.10643959552953698No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)