FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13551
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCG5033.7119031805770795No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCT3442.538558039997048No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3432.5311785108110105No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1901.4021105453472067No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG940.6936757434875654No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC880.6493985683713379No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTAT840.6198804516271862No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT740.5460851597668068No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGT710.5239465722086931No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGG640.4722898679064276No Hit
ATTGCGCAATGTGATGACGGGGGTTTCTGAAACCGGACGCCTACAAGCAG640.4722898679064276No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.4722898679064276No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT600.44277175116227585No Hit
ATTGCGCAATGCGCTAACGGGGATGCGCTGTCACGGCAGCGCCGGCTGAG580.42801269279019993No Hit
ATTGCGCAATGGCAGGACCGGGGATGTCGGCTCGTCGCATCCTGGGGCTG530.39111504686001036No Hit
ATTGCGCAATGTAATCACCGGGACACAATTGACCCTGTTGAAAAGGTCCT500.36897645930189654No Hit
ATTGCGCAATGGTATTAAGGGGAGGAGGGTCTTGAGCTTGAGGAATCGGA460.3394583425577448No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT450.3320788133717069No Hit
ATTGCGCAATGTTGGTGTCGGGCAGCGTGGCCGCGTCGTTGTCGATGACC450.3320788133717069No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG450.3320788133717069No Hit
ATTGCGCAATGTGCGGCGAGGGGGTTCCAGGAAATAGCCCCTCCATTATA450.3320788133717069No Hit
ATTGCGCAATGAACAACGGGGGAAGGCCCCTAACGAGACCCGTGGCCAGA440.32469928418566896No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGGTATCG440.32469928418566896No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC440.32469928418566896No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT430.317319754999631No Hit
ATTGCGCAATGTCTAGTCCGGGAAGGCCGGCCGTGCCTACCACAAGTACC410.30256069662755514No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG370.2730425798834034No Hit
ATTGCGCAATGCGGTATGTGGGGAAAAATTGTCGGATGAGTTGTGTTTAG370.2730425798834034No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG360.2656630506973655No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA340.25090399232528965No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGA300.22138587558113793No Hit
ATTGCGCAATGATACTGAGGGGATTGAGGGTGGTGAATACGGTGACCGTG300.22138587558113793No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC300.22138587558113793No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT300.22138587558113793No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC290.21400634639509997No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCGCG280.20662681720906204No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGG280.20662681720906204No Hit
ATTGCGCAATGCCTCTTTCGGGCGGGTGCTTACACCCCTATCTGCCACCA280.20662681720906204No Hit
ATTGCGCAATGCAAGTCGGGGGGAAGTACCAGGCAGTGACAGCCACTCTG270.19924728802302416No Hit
ATTGCGCAATGGCAGTGGGGGGTAGAATTCCACGTGTAGCAGTGAAATGC270.19924728802302416No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG270.19924728802302416No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC250.18448822965094827No Hit
ATTGCGCAATGCAAGGCAGGGGAAGATGAGCTACGTCGCCAAGGACAAGG250.18448822965094827No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC250.18448822965094827No Hit
ATTGCGCAATGTTAATACAGGGAAGGTCGTTGTGATCGACTTTTGGGCTA230.1697291712788724No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG220.16234964209283448No Hit
ATTGCGCAATGCAAGTGAGGGGAAGATGAGCTACGTCGCCAAGGACAAGG220.16234964209283448No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT220.16234964209283448No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT220.16234964209283448No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCG200.14759058372075862No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC180.13283152534868276No Hit
ATTGCGCAATGGTAGAGAGGGGGGACCGCATAGACCATGTCGAGTGCATT180.13283152534868276No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT180.13283152534868276No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG180.13283152534868276No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCG170.12545199616264482No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT170.12545199616264482No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC160.1180724669766069No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT150.11069293779056896No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT150.11069293779056896No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCTCG150.11069293779056896No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTCT140.10331340860453102No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT140.10331340860453102No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)