FastQCFastQC Report
Tue 7 May 2024
EGAF00007822130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10800
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTA9889.148148148148149No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3833.546296296296296No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2552.361111111111111No Hit
CTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGG1361.2592592592592593No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGTCGCC1201.1111111111111112Illumina RNA PCR Primer (100% over 22bp)
CTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTC930.8611111111111112No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTG580.537037037037037No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCC570.5277777777777778No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGAC510.4722222222222222No Hit
ATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGG460.42592592592592593No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA440.4074074074074074No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.3703703703703704No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC390.3611111111111111No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG380.35185185185185186No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGGCGCC370.3425925925925926Illumina RNA PCR Primer (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGA370.3425925925925926No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.3148148148148148No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG320.2962962962962963No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC300.2777777777777778No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT300.2777777777777778No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG300.2777777777777778No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC290.2685185185185185No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC250.23148148148148145No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA240.2222222222222222No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG230.21296296296296297No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGTGGCC220.2037037037037037Illumina RNA PCR Primer (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.19444444444444445No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT200.1851851851851852No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGA190.17592592592592593No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGTCGGC170.1574074074074074Illumina RNA PCR Primer (95% over 22bp)
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACA170.1574074074074074No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA160.14814814814814814No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG160.14814814814814814No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTC150.1388888888888889No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA140.12962962962962962No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAA140.12962962962962962No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGAC130.12037037037037038No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC130.12037037037037038No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC130.12037037037037038No Hit
ATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGG120.1111111111111111No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1111111111111111No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC110.10185185185185185No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA110.10185185185185185No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA110.10185185185185185No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA110.10185185185185185No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA110.10185185185185185No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA110.10185185185185185No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)