FastQCFastQC Report
Tue 7 May 2024
EGAF00007822131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11512
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4483.891591382904795No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCG3312.875260597637248No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2572.2324530924252954No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCT2382.0674079221681723No Hit
ATTGCGCAATGGGCTAAGGGGGCAGAACCTTACCATCCCTTGACATGGCA1321.1466296038915913No Hit
ATTGCGCAATGGTTGGGTGGGGGTACCGGGGCTAAAGCCATACACCGAAG1120.9728978457261988No Hit
ATTGCGCAATGCTAGCAGGGGGTGAAAAGGTAGGACAAAAGAAGACTAAC1070.9294649061848506No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG760.660180681028492No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC640.5559416261292565No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCGATATATCG630.5472550382209868No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATA620.5385684503127172No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAC600.5211952744961779No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCG540.4690757470465601No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG530.4603891591382905No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATAT530.4603891591382905No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT510.4430159833217512No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.4343293954134816No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT470.4082696316886727No Hit
ATTGCGCAATGCATTATCCGGGGGAAAACTTGGATGTCTGATGGGAAAGG460.39958304378040305No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCGATATATCT410.3561501042390549No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT410.3561501042390549No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT380.330090340514246No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG330.2866574009728978No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC290.25191104933981934No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT280.2432244614315497No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG270.23453787352328004No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA270.23453787352328004No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT270.23453787352328004No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG240.20847810979847115No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGAT230.19979152189020152No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC230.19979152189020152No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT230.19979152189020152No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT210.18241834607366228No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC200.17373175816539263No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG190.165045170257123No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCT190.165045170257123No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG190.165045170257123No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC180.1563585823488534No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCCT180.1563585823488534No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACA170.14767199444058374No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC170.14767199444058374No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC170.14767199444058374No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA160.13898540653231412No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG160.13898540653231412No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT160.13898540653231412No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT150.13029881862404447No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTG140.12161223071577484No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTCT130.11292564280750521No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT130.11292564280750521No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC130.11292564280750521No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG130.11292564280750521No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT120.10423905489923557No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCCTGT120.10423905489923557No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAC120.10423905489923557No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGCTGTCTCTTA120.10423905489923557No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)