FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11512
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTA8237.149061848505907No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5825.055594162612926No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3402.953439888811675No Hit
CTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGG1731.5027797081306462No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGTCGCC1371.1900625434329395Illumina Single End PCR Primer 1 (95% over 22bp)
CTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTC990.8599722029186935No Hit
ATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGG770.6688672689367616No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTG720.6254343293954135No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCC700.6080611535788742No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC650.564628214037526No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.5038220986796387No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGAC560.4864489228630994No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG520.45170257123002083No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC400.34746351633078526No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG400.34746351633078526No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC330.2866574009728978No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA310.2692842251563586No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG300.26059763724808893No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.2171646977067408No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTC240.20847810979847115No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGA230.19979152189020152No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC230.19979152189020152No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG200.17373175816539263No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGGCGCC200.17373175816539263Illumina RNA PCR Primer (95% over 22bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA200.17373175816539263No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT200.17373175816539263No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA180.1563585823488534No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGA180.1563585823488534No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGGGGTCGCC180.1563585823488534Illumina RNA PCR Primer (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA170.14767199444058374No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA160.13898540653231412No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATGGACACTCCGTGTAGATC150.13029881862404447No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA150.13029881862404447No Hit
TGTGTAGCCCATCCCGTAAGGGCCATGAGGACTTGACGTCATCCACACCT150.13029881862404447No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC150.13029881862404447No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC140.12161223071577484No Hit
CATCTGACGCTGCCGACGATGGACACTCCGTGTAGATCTCGGTGGTCGGC140.12161223071577484Illumina RNA PCR Primer (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA140.12161223071577484No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGACA140.12161223071577484No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA130.11292564280750521No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC130.11292564280750521No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA120.10423905489923557No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT120.10423905489923557No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA120.10423905489923557No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT120.10423905489923557No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)