FastQCFastQC Report
Tue 7 May 2024
EGAF00007822141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15878
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC6614.16299282025444No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTCTATCG5963.753621362892052No Hit
ATTGCGCAATGAATGCATGGGGAAGGAAAAGAAGGAAGAGCAGGTCATCA4132.601083259856405No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC4072.563295125330646No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTCTATCT4052.5506990804887266No Hit
ATTGCGCAATGAATGGGGGGGGAATAGGAGTAGGAAAGATGAGTCATGGT3752.3617584078599316No Hit
ATTGCGCAATGGATACGTGGGGGGGGGAATAGGAGTAGGAAAGATGAGTC2521.5871016500818742No Hit
ATTGCGCAATGCAGGGCAGGGGGCACTGTTGAGGCGGGTGCCTTTCCACC2391.5052273586093967No Hit
ATTGCGCAATGCTATCAACGGGGCTTTCTCTTCTCCATTCCTCCGCCATC2221.3981609774530799No Hit
ATTGCGCAATGCGAGGGAGGGGATATGCTTTGGGTAGCTGTAAGTAAGCG1741.0958559012470084No Hit
ATTGCGCAATGATACCTGAGGGGCAAAGAAAGAGAAAAGAAAGAAAAAAA1621.0202796321954906No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.8439350044086157No Hit
ATTGCGCAATGGTGTGGGGGGGGAATAGGAGTAGGAAAGATGAGTCATGG1150.7242725784103792No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTC1030.6486963093588614No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTCTAT840.5290338833606247No Hit
ATTGCGCAATGTAGGCAGGGGAAGTTTCCAAGGGCGCATGGTGGATGCCT640.40307343494142844No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG620.3904773900995087No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCAC600.3778813452575891No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG600.3778813452575891No Hit
ATTGCGCAATGGGCCGCCTGGGCCAAAACCACAAACGAACTAAAGCCCAA600.3778813452575891No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT540.3400932107318302No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC530.3337951883108704No Hit
ATTGCGCAATGTGGACGTGGGGGGAAATTCGAAAGAAAGCTCAAAGGAAA460.28970903136415166No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT450.2834110089431918No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC420.26451694168031237No Hit
ATTGCGCAATGTCAGCACAGGGAGGAGATTTAAAGATGTAAGGAGATAAA350.22043078473359368No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTCTATCG350.22043078473359368No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA320.20153671747071422No Hit
ATTGCGCAAGTGTTCGTCGGGGGATTGGTTGGGCGGTGAAGGGGTCCCAG300.18894067262879455No Hit
ATTGCGCAATGAGCAAACGGGGGATTCCGTGGGTGGTGGTGCATGGCCGT270.1700466053659151No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACT270.1700466053659151No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT260.16374858294495528No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC260.16374858294495528No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC250.15745056052399548No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG250.15745056052399548No Hit
ATTGCGCAATGAGGAGCCGGGGGAGCTTAAATTTGAGAAGAGGACATCCT240.15115253810303564No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT230.14485451568207583No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCAC220.13855649326111602No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCA220.13855649326111602No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC210.13225847084015618No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTCTATCGCG210.13225847084015618No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT200.12596044841919637No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT200.12596044841919637No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACGGTTCTATCG200.12596044841919637No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTT190.11966242599823655No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG190.11966242599823655No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA170.10706638115631692No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT170.10706638115631692No Hit
ATTGCGCAATGACGTCAAGGGGCATCTAAGCGGGAAACTCGTTTCAAGAT170.10706638115631692No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)