FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11939
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCG4844.053940866069185No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4673.9115503811039454No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCT3452.8896892537063406No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2772.3201273138453806No Hit
ATTGCGCAATGTGCTTGTTGGGGCAGGGGTTGGGGAAAGACGCGCAGAAA1551.2982661864477762No Hit
ATTGCGCAATGCCACGCTGGGGGTCCGATCGGGCAATGGTGCGAAGCTAT1421.189379345003769No Hit
ATTGCGCAATGGGTCGGTAGGGGGTGGTAATCGAGTGGTTGGCTAGCGTG1140.9548538403551385No Hit
ATTGCGCAATGCTTCCCTTGGGGAACATTGTAGTTCAGTCTTTGCAAGAT1140.9548538403551385No Hit
ATTGCGCAATGTATACCCGGGGGTGAAGAGCCTCGAGAAGATGAGCAATG1100.9213501968339057No Hit
ATTGCGCAATGTGCCTTCTGGGAGAACCTGCAGGAGACGAACCCTGACCG920.7705838009883574No Hit
ATTGCGCAATGGATAGACCGGGGACGAGGGGAGTGAAACAGTACCTGAAA910.7622078901080492No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.6533210486640422No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTG760.6365692269034258No Hit
ATTGCGCAATGAATTAATAGGGGAAGGTTGAGGCATGATGGGGAGCCCAT630.5276823854594187No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG590.4941787419381858No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCAT470.39366781137448703No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACC450.3769159896138705No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC450.3769159896138705No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG370.3099087025714046No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC350.29315688081078817No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT340.28478096993047997No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCG310.25965323728955525No Hit
ATTGCGCAATGCACGTCCGGGGATTGAGGCCATTGACGAGGGTTCGATCG300.251277326409247No Hit
ATTGCGCAATGAACGGATCGGGAGAAATGGCTTGTCATCGAGAAACGCAT260.21777368288801408No Hit
ATTGCGCAATGCACTTCTGGGGGGTGGATGCCTTGGCGATATCAGGCGAT260.21777368288801408No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT240.20102186112739762No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCT240.20102186112739762No Hit
ATTGCGCAATGATGTGTACGGGATGATTCGCGCGATGCAAGAGAGAAACA220.18427003936678113No Hit
ATTGCGCAATGAACACACAGGGGGGTCCAGGCCAAGTCCATAATGGATCC220.18427003936678113No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT210.1758941284864729No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC210.1758941284864729No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCAGCG200.16751821760616467No Hit
ATTGCGCAATGTGAGCGTGGGGAGAAATGGCTTGTCATCGAGAAACGCAT200.16751821760616467No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC200.16751821760616467No Hit
ATTGCGCAATGCCAGTAACGGGGTGGGAACAAGAAAAACAAGCCAGCCAA200.16751821760616467No Hit
ATTGCGCAATGTGAAGGCAGGGCAGGGCAGGGCAGAGTGGTCGAAGAGGC180.1507663958455482No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG180.1507663958455482No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATT170.14239048496523998No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT170.14239048496523998No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACC170.14239048496523998No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA170.14239048496523998No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA160.13401457408493173No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA160.13401457408493173No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT160.13401457408493173No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCT160.13401457408493173No Hit
ATTGCGCAATGCCAAGCCCGGGATACCAAGGCGCTTGAGAGAACTATGTT150.1256386632046235No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG150.1256386632046235No Hit
ATTGCGCAATGATAGGGTAGGGGAACAAAACGGCGAAAGCAAGCGAACAA150.1256386632046235No Hit
ATTGCGCAATGCATCTTCCGGGAAGGGCGAGGACTTTGAGCCGTGCAAGC150.1256386632046235No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC140.11726275232431527No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC140.11726275232431527No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC140.11726275232431527No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCGATTGCATCG140.11726275232431527No Hit
ATTGCGCAATGAAGGAAGGGGGGAAGTTGATCAAGCTGTATCCCGCCATC140.11726275232431527No Hit
ATTGCGCAATGTATACTCCGGGCGTGGTATTGCTGCTGCTGGTACGATTG130.10888684144400704No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCGCG130.10888684144400704No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT130.10888684144400704No Hit
ATTGCGCAATGCACAAGAGGGGGGGCCACTACCTCGGCGAGTTTGCCATT130.10888684144400704No Hit
ATTGCGCAATGGGGGGAGGGGGAAGCCGCGTGCAGCCGGAAGGGGTAGCG120.10051093056369881No Hit
ATTGCGCAATGCTGAGTATGGGATGGTGCTTGAGAATGTCAAGGAGATGT120.10051093056369881No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC120.10051093056369881No Hit
ATTGCGCAATGGGGGAGAGGGGGATGACAGGACAACACGAATGAACGAAC120.10051093056369881No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)