FastQCFastQC Report
Tue 7 May 2024
EGAF00007822147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12532
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCG5574.444621768273221No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCT3983.1758697733801466No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2381.899138206192148No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1391.109160548994574No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC910.7261410788381742No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGG830.6623045004787743No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG800.6383657835939993No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTAT790.6303862112990744No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGT750.5984679221193745No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT730.5825087775295245No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT700.5585700606447495No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.5266517714650495No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT570.4548356208107246No Hit
ATTGCGCAATGACCCCACAGGGATTGACGGGGACCCGCACAAGCGGTGGA500.39897861474624957No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG480.3830194701563996No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT470.3750398978614746No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA440.3511011809766997No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG430.34312160868177466No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC420.33514203638684964No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGG410.3271624640919247No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGGTATCG400.31918289179699966No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC300.23938716884774977No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT250.19948930737312479No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC250.19948930737312479No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCGCG240.1915097350781998No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG240.1915097350781998No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT240.1915097350781998No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG240.1915097350781998No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT220.17555059048834984No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCG210.16757101819342482No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC210.16757101819342482No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT210.16757101819342482No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC200.15959144589849983No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGA190.15161187360357486No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT190.15161187360357486No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC190.15161187360357486No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG190.15161187360357486No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT180.14363230130864987No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCTCG180.14363230130864987No Hit
ATTGCGCAATGAGGGTACAGGGGCTCTTCGGACTTGGGCTTGGGTTCCGG180.14363230130864987No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT170.13565272901372485No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGGTATCT160.12767315671879986No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTAGCG160.12767315671879986No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG150.11969358442387489No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGTC140.11171401212894988No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTCT140.11171401212894988No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC140.11171401212894988No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCG140.11171401212894988No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT140.11171401212894988No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGC140.11171401212894988No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT130.1037344398340249No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT130.1037344398340249No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG130.1037344398340249No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG130.1037344398340249No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)