FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12532
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3082.4577082668368977No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGTA2782.218321097989148No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGGT2782.218321097989148No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGTT2251.795403766358123No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2011.6038940312799232No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGGA1801.4363230130864986No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCA800.6383657835939993No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGG740.5904883498244494No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTG740.5904883498244494No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG570.4548356208107246No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.4468560485157995No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCA450.35908075327162464No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG410.3271624640919247No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC410.3271624640919247No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.3112033195020747No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG390.3112033195020747No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGGG380.30322374720714973No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA380.30322374720714973No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGTG370.2952441749122247No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG370.2952441749122247No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA290.23140759655282478No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC250.19948930737312479No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC240.1915097350781998No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC220.17555059048834984No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTC220.17555059048834984No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA210.16757101819342482No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT200.15959144589849983No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT190.15161187360357486No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA190.15161187360357486No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAG180.14363230130864987No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAA170.13565272901372485No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA170.13565272901372485No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA160.12767315671879986No Hit
CATCTGACGCTGCCGACGATGTCAAGAGGGTGTAGATCTCGGTGGTCGCC150.11969358442387489Illumina Single End PCR Primer 1 (100% over 21bp)
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT140.11171401212894988No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA140.11171401212894988No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAA140.11171401212894988No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGTA140.11171401212894988No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAA140.11171401212894988No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGGGGT140.11171401212894988No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA130.1037344398340249No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGTA130.1037344398340249No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA130.1037344398340249No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC130.1037344398340249No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)