FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11490
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCG4323.759791122715405No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4283.724978241949521No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCT3903.3942558746736298No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3042.645778938207137No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCGATATATCG1060.9225413402959095No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG900.7832898172323759No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAC680.5918189730200174No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATAT610.5308964316797214No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.5221932114882507No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT590.5134899912967797No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC590.5134899912967797No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATA590.5134899912967797No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT580.504786771105309No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT460.40034812880765885No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG440.38294168842471715No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT420.36553524804177545No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCG410.3568320278503046No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG400.34812880765883375No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCGATATATCT360.3133159268929504No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC360.3133159268929504No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC350.30461270670147955No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC280.24369016536118365No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG280.24369016536118365No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC280.24369016536118365No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT270.2349869451697128No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG250.2175805047867711No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG250.2175805047867711No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA240.20887728459530025No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAC240.20887728459530025No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT240.20887728459530025No Hit
ATTGCGCAATGCTTATCGGGGGGGCGTGAAGGTTGAGGCATGATGGGGAG230.20017406440382943No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG220.19147084421235858No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCTCG210.18276762402088773No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT200.17406440382941687No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCT200.17406440382941687No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT190.16536118363794605No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA190.16536118363794605No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC190.16536118363794605No Hit
ATTGCGCAATGACGGGGCCGGGGCCTGTGGCAGACGGTGTCGCGCGGCAT180.1566579634464752No Hit
ATTGCGCAATGGACAGCGGGGGGGCCAGAGCCAGAATCATGCAGATCCAC180.1566579634464752No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT160.1392515230635335No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC160.1392515230635335No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACA160.1392515230635335No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT160.1392515230635335No Hit
ATTGCGCAATGTGGACGGCGGGCACTGTTGGAAACGAAGTTTTGTAATTT160.1392515230635335No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTG150.13054830287206268No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGACCGATATATCG150.13054830287206268No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT150.13054830287206268No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGAT140.12184508268059183No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCGCG140.12184508268059183No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCTG130.11314186248912096No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGATG130.11314186248912096No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCCTGT120.10443864229765012No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG120.10443864229765012No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTCT120.10443864229765012No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC120.10443864229765012No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT120.10443864229765012No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC120.10443864229765012No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTG120.10443864229765012No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCCTGTC120.10443864229765012No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)