FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9969
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCG4624.634366536262414No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3713.7215367639683015No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCT3103.109639883639282No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2492.497743003310262No Hit
ATTGCGCAATGACAACGCGGGGAAATAACAGAAAGGCTCCTCCCCCTAAT960.9629852542882937No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCAC760.7623633263115659No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG690.6921456515197111No Hit
ATTGCGCAATGAGAGTGAGGGCGCTGGCGGCGTGCTTAACACATGCAAGT640.6419901695255291No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.61189688032902No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT590.5918346875313472No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAAT510.5115859163406561No Hit
ATTGCGCAATGATTCTACGGGGGTTCTGCAAGATCATCTGAAAAGGATTT500.5015548199418196No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC480.48149262714414687No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT420.4213060487511285No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT370.3711505667569465No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCG340.34105727756043736No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT300.30093289196509176No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT290.2909017955662554No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC280.280870699167419No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG270.2708396027685826No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC260.26080850636974623No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG260.26080850636974623No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC220.2206841207744006No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA220.2206841207744006No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG220.2206841207744006No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCT210.21065302437556424No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG200.20062192797672784No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATGG180.18055973517905505No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTC180.18055973517905505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC170.17052863878021868No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT170.17052863878021868No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC160.16049754238138228No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT160.16049754238138228No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG160.16049754238138228No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT150.15046644598254588No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC150.15046644598254588No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCGCG140.1404353495837095No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC140.1404353495837095No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAAGCG140.1404353495837095No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCA140.1404353495837095No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT140.1404353495837095No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAAGCT130.13040425318487311No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT130.13040425318487311No Hit
ATTCGCTATGGAATCCTCGGGGGGTCTCGTAATTGGAATGAGAACAATTT130.13040425318487311No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT120.12037315678603672No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT120.12037315678603672No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG110.1103420603872003No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC110.1103420603872003No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTG110.1103420603872003No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT110.1103420603872003No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT110.1103420603872003No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC110.1103420603872003No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGGTGCCT100.10031096398836392No Hit
ATTGCGCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTC100.10031096398836392No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGATG100.10031096398836392No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)