FastQCFastQC Report
Tue 7 May 2024
EGAF00007822162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9969
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4694.704584211054268No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3043.0494533052462636No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGTA2262.267027786137025No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGGT2162.166716822148661No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGTT1791.7955662553917142No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGGA1441.4444778814324404No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGG790.792456615508075No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCA540.5416792055371652No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTG480.48149262714414687No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.3711505667569465No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC340.34105727756043736No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG310.3109639883639282No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG310.3109639883639282No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG300.30093289196509176No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC290.2909017955662554No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.280870699167419No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA260.26080850636974623No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT260.26080850636974623No Hit
GTTCTTCTCCCAGCCTCTGCCCTCCTTCTGTCTCCTGGGTGGTCTCCCGC250.2507774099709098No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGTG240.24074631357207343No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC220.2206841207744006No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG220.2206841207744006No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.21065302437556424No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA210.21065302437556424No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTC190.19059083157789147No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC180.18055973517905505No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC180.18055973517905505No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA170.17052863878021868No Hit
GCCATTTCCCGGAGACTTTTCCTTTCCTCTCGACAGTAATTGAGATCTTC150.15046644598254588No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAG140.1404353495837095No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGTGGG130.13040425318487311No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCA130.13040425318487311No Hit
CCAACAAGCTGATAGGCCGCGAGTCCATCCAAAACCGATAAATCTTTCCA130.13040425318487311No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC120.12037315678603672No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAAGAGGGGGGA120.12037315678603672No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGA110.1103420603872003No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGTA110.1103420603872003No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA110.1103420603872003No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.1103420603872003No Hit
CATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGTCAA100.10031096398836392No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA100.10031096398836392No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA100.10031096398836392No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAT100.10031096398836392No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)