FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16237
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC7244.45895177680606No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCG6924.26187103528977No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC5593.4427542033626897No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCT4222.599002278746074No Hit
ATTGCGCAATGGAGTCATCGGGGGTGCTGGAAGGTTAAGGGGACCTGTTA3882.3896039908850155No Hit
ATTGCGCAATGTGTGGATGGGGGGGTCAAGTGAATAAGAGCACGTGGTGG2911.7922029931637617No Hit
ATTGCGCAATGGAGACCCCGGGGCTCTTTCCAGCCAGCGCCGAGCGATGG2471.521216973578863No Hit
ATTGCGCAATGACATTCGTGGGGGTTCCAGGAAATAGCCCCTCCATTATA2201.3549300979244934No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1891.1640081295805875No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCAC1641.010038800270986No Hit
ATTGCGCAATGGATCAACCGGGCGCTGGCGGCGTGCTTAACACATGCAAG890.5481308123421814No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAAT850.5234957196526452No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG810.49886062696310896No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG800.4927018537907249No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC720.4434316684116524No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCG640.3941614830325799No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT630.38800270986019586No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT520.32025620496397117No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTC500.30793865861920305No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT490.301779885446819No Hit
ATTGCGCAATGGGAGAAGGGGGGAGAAACGAAACAAGAAAAAATAAAAAA430.26482724641251465No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT420.25866847324013054No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG380.2340333805505943No Hit
ATTGCGCAATGAGCGAGTGGGGCGAGAAGGTCGCCGAGGCGACGGCCGCC340.20939828786105807No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC330.203239514688674No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG330.203239514688674No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC320.19708074151628996No Hit
ATTGCGCAATGCACCCTGGGGGGGAGCCCAGCAGCGGTCCTACACGGGCG320.19708074151628996No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG310.1909219683439059No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCGCG300.18476319517152184No Hit
ATTGCGCAATGTGACCCACGGGGGGTGCTCCGCGTCGAAGGCGCGCGGGG290.17860442199913776No Hit
ATTGCGCAATGAGATGGCCGGGGGGCGAGGAGCGGGGACGACGCAGGCCC290.17860442199913776No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT280.17244564882675373No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA270.16628687565436964No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC260.16012810248198558No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC250.15396932930960153No Hit
ATTGCGCAATCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA250.15396932930960153No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC230.1416517829648334No Hit
ATTGCGCAATGGGAGGGCGGGGCCGGTGAGGGTGAGGCCCGCGCGGGTGC230.1416517829648334No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAAGCG220.13549300979244935No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA220.13549300979244935No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCT210.12933423662006527No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC210.12933423662006527No Hit
ATTGCGCAATGGTGGGTACGGGCAGACTAGAGTGCAGTAGGGGAGACTGG210.12933423662006527No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCA210.12933423662006527No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC200.12317546344768122No Hit
ATTGCGCAATGTGCAGGTCGGGAGGGTTACTGAATTCAGATAAACTCCGA200.12317546344768122No Hit
ATTGCGCAATGGATCGGTAGGGCATAGAAAACTAGATAAACAAAAAAAAC190.11701669027529715No Hit
ATTGCGCAATGTATGCTCTGTCTCTTATACACATCTCCGAGCCCACGAGA190.11701669027529715No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT190.11701669027529715No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT190.11701669027529715No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT180.1108579171029131No Hit
ATTGCGCAATGTAAGCCCTGGGGCAATTGCCGGATGGAAAACGGTTAACG180.1108579171029131No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG180.1108579171029131No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT170.10469914393052904No Hit
ATTGCGCAATGTAACGCACGGGGGAGCAAAAAAGAACAAACAAATAAAAA170.10469914393052904No Hit
ATTGCGCAATGTCGTTGTCGGGATGGCGACGACCCACATGAAGAGCACCC170.10469914393052904No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)