FastQCFastQC Report
Tue 7 May 2024
EGAF00007822164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16237
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT9245.690706411282872No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC6604.064790293773481No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGTA3722.291063620126871No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGGT3101.909219683439059No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGTT2671.6443924370265441No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGGA2161.3302950052349571No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATA1681.0346738929605221No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.714417687996551No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC1090.6713062757898627No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTG820.505019400135493No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.34489129765350746No Hit
GGCACTGTCCCTGACCCGGATCACGGGCCGAAGTTAGATATCCAGAGTGA410.2525097000677465No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGGG390.24019215372297836No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAC370.22787460737821028No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG370.22787460737821028No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACG340.20939828786105807No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.20939828786105807No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC330.203239514688674No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG320.19708074151628996No Hit
GTACCACACAGGTCATTTCACGTACGGGGCTATCACCCGCTATGGCCAGC310.1909219683439059No Hit
ATCCTACACAAGTCACACCGAATACCAATACCAAACTATAGTAAAGGTCA290.17860442199913776No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC280.17244564882675373No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC280.17244564882675373No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG270.16628687565436964No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG250.15396932930960153No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA250.15396932930960153No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAATAGTGTG250.15396932930960153No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA250.15396932930960153No Hit
CTATAGTAAAGGTCACGGGGTCTTTCCGTCCTTCTGCGCGTAACGAGCAT240.14781055613721747No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT230.1416517829648334No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA220.13549300979244935No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC220.13549300979244935No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.12933423662006527No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACGAAT210.12933423662006527No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC210.12933423662006527No Hit
AGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA200.12317546344768122No Hit
GTATTTCAACGGCGACTCCACGAACACTGGCGTGCCCGCTTCACAGTCTC200.12317546344768122No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAC190.11701669027529715No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC190.11701669027529715No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.11701669027529715No Hit
GCCCTATACGTCGTCTTGAAGCCGACTTAGCAGAGCCCTGTGTTTTTGCT190.11701669027529715No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC180.1108579171029131No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAA180.1108579171029131No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.10469914393052904No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)