FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9571
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTA7998.348134991119005No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5856.112213979730436No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3183.322536830007314No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGTCGCC2222.3195068435900112Illumina Single End PCR Primer 1 (95% over 23bp)
CTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGG2152.24636924041375No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTC1071.1179605056942847No Hit
ATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGG660.6895831156618953No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTG540.5642043673597325No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC470.4910667641834709No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTT460.4806185351582907No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGG440.45972207710793017No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.4179291610072093No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG350.3656880158813081No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.3656880158813081No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG310.32389509978058717No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGA290.3029986417302267No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG270.2821021836798662No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG200.20896458050360464No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC200.20896458050360464No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC190.19851635147842442No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC180.1880681224532442No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTG180.1880681224532442No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT160.16717166440288372No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTT160.16717166440288372No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGTCGGC160.16717166440288372Illumina Single End PCR Primer 1 (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC160.16717166440288372No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA160.16717166440288372No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGGCGCC140.14627520635252325No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG140.14627520635252325No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA130.135826977327343No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACT130.135826977327343No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA130.135826977327343No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA120.12537874830216278No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGGGGTCGCC110.11493051927698254No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATC110.11493051927698254No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA100.10448229025180232No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA100.10448229025180232No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA100.10448229025180232No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT100.10448229025180232No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA100.10448229025180232No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)