FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13212
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCG4833.6557674841053585No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCT3632.7475022706630337No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3452.611262488646685No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1921.4532243415077202No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT900.6811989100817438No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG860.6509234029669997No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC830.6282167726309416No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGT750.5676657584014532No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTAT680.5146836209506509No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT660.49954586739327883No Hit
ATTGCGCAATGCACAGGGCGGGGGATCAAGCGACTAAGTGCATATGGTGG640.48440811383590676No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGG610.46170148349984863No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT480.36330608537693004No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT470.35573720859824404No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.348168331819558No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC450.3405994550408719No Hit
ATTGCGCAATGAGACTCCAGGGGTGAAAAATGGAAAGACGAGAGAGAGAG440.3330305782621859No Hit
ATTGCGCAATGCGCTTACGGGGCGAGCCCTCCCTCAATAGAGATATAATA410.3103239479261278No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG400.3027550711474417No Hit
ATTGCGCAATGAGCGGACTGGGGCAACATGAATACATAGTGTTGCAAGGC380.28761731759006964No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC350.26491068725401146No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGA340.25734181047532545No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG340.25734181047532545No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC330.24977293369663942No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC320.24220405691795338No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGGTATCG320.24220405691795338No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA310.23463518013926732No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT290.21949742658189525No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT270.20435967302452315No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG260.19679079624583712No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGG230.174084165909779No Hit
ATTGCGCAATGGGAGGTATGGGGATGCGTTCGCCGCTGGCGCGCATGCAG230.174084165909779No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG230.174084165909779No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT220.16651528913109295No Hit
ATTGCGCAATGAGGGTATGGGGATGCGTTCGCCGCTGGCGCGCATGCAGG220.16651528913109295No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCG210.15894641235240692No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG200.15137753557372086No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCGCG190.14380865879503482No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTCT190.14380865879503482No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCG190.14380865879503482No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC190.14380865879503482No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTG170.12867090523766272No Hit
ATTGCGCAATGTGGTAATAGGGATATAAAAAGCCGCAGGATGAACACTGC170.12867090523766272No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG170.12867090523766272No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG160.12110202845897669No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT160.12110202845897669No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT160.12110202845897669No Hit
ATTGCGCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGA150.11353315168029066No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT150.11353315168029066No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC150.11353315168029066No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT140.1059642749016046No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGGTATCT140.1059642749016046No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT140.1059642749016046No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTAGCG140.1059642749016046No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)