FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13212
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTA9046.842264607932183No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4783.6179231002119283No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGTCGCC2882.17983651226158Illumina Single End PCR Primer 1 (95% over 23bp)
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2852.1571298819255222No Hit
CTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGG1741.3169845594913716No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTC1170.8855585831062671No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGG780.5903723887375113No Hit
ATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGG750.5676657584014532No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTG690.5222524977293369No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.48440811383590676No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTT610.46170148349984863No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG480.36330608537693004No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG410.3103239479261278No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG400.3027550711474417No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC400.3027550711474417No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG370.2800484408113836No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA320.24220405691795338No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC320.24220405691795338No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC310.23463518013926732No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC310.23463518013926732No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.2270663033605813No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGA280.2119285498032092No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC260.19679079624583712No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGGCGCC240.18165304268846502No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTT230.174084165909779No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA220.16651528913109295No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.15894641235240692No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA210.15894641235240692No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA210.15894641235240692No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACT200.15137753557372086No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC200.15137753557372086No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTG190.14380865879503482No Hit
CATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTC170.12867090523766272No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA170.12867090523766272No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGTCGGC160.12110202845897669Illumina Single End PCR Primer 1 (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA160.12110202845897669No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATC160.12110202845897669No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTC150.11353315168029066No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA140.1059642749016046No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT140.1059642749016046No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA140.1059642749016046No Hit
ATCTATCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTA140.1059642749016046No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)