FastQCFastQC Report
Tue 7 May 2024
EGAF00007822169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12503
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCG5514.406942333839878No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCT3562.8473166440054385No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3332.663360793409582No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1971.5756218507558186No Hit
ATTGCGCAATGACAGAGGAGGGATACCCTAATAGTCCAAGCAGACAATGA820.6558425977765336No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTC810.6478445173158441No Hit
ATTGCGCAATGCGGCCGTGGGGGCCGAGAATGGCTGCAAGTGCGGAGCGA810.6478445173158441No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG800.6398464368551547No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAAT770.6158521954730865No Hit
ATTGCGCAATGTAATGGGAGGGGGTGCTTCCGAGTCTGATAGATTACATC690.551867551787571No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAG670.5358713908661921No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.5278733104055027No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCG540.4318963448772295No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC510.40790210349516115No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT450.35991362073102456No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT420.33591937934895627No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC410.3279212988882668No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT400.31992321842757737No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC320.2559385747420619No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATGG310.2479404942813725No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG280.2239462528993042No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA260.2079500919779253No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT250.19995201151723585No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT240.19195393105654643No Hit
ATTGCGCAATGGCATATCCGGGCACAAAGGCAAGAAAAGGGGGGAAAAAG240.19195393105654643No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGC230.183955850595857No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT220.17595777013516756No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG220.17595777013516756No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG210.16795968967447814No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG210.16795968967447814No Hit
ATTGCGCAATGGGGCAACAGGGGGAGACTCTGTCTCAAAAAAACAGAAAA210.16795968967447814No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT200.15996160921378869No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAAGCG190.15196352875309926No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA180.14396544829240981No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCGCG180.14396544829240981No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAAGCT170.1359673678317204No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT160.12796928737103094No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGT150.11997120691034152No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC150.11997120691034152No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC150.11997120691034152No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT140.1119731264496521No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT140.1119731264496521No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT140.1119731264496521No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAG130.10397504598896265No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)