FastQCFastQC Report
Tue 7 May 2024
EGAF00007822176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6927
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTA4426.380828641547567No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3745.399162696694096No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2032.9305615706655117No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGTCGCC1892.728453876136856Illumina Single End PCR Primer 1 (95% over 23bp)
CTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGG1552.23762090371012No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTC801.15490111159232No Hit
ATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGG560.8084307781146238No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.7362494586401039No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGG370.534141764111448No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC360.5197055002165439No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTT350.50526923632164No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG280.4042153890573119No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.4042153890573119No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG270.3897791251624079No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG270.3897791251624079No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTG250.3609065973726No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGGCGCC240.34647033347769596No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG240.34647033347769596No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACT160.23098022231846396No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGA160.23098022231846396No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGGGGTCGCC150.21654395842356No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA150.21654395842356No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC140.20210769452865596No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT140.20210769452865596No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.20210769452865596No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT130.187671430633752No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC130.187671430633752No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA130.187671430633752No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.17323516673884798No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA110.158798902843944No Hit
CTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGTTCTCGG110.158798902843944No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATC110.158798902843944No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA100.14436263894904No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGTCGGC100.14436263894904Illumina Single End PCR Primer 1 (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC100.14436263894904No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA100.14436263894904No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAA90.12992637505413598No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA90.12992637505413598No Hit
TATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTG90.12992637505413598No Hit
ATCTATCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTA90.12992637505413598No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCGGGGTGTA80.11549011115923198No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA80.11549011115923198No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA80.11549011115923198No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC80.11549011115923198No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA70.10105384726432798No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA70.10105384726432798No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTT70.10105384726432798No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT70.10105384726432798No Hit
CCCTAAGCTTCGGCTCGTGGCTTGAGCCCCGTTACATCTTCGGCGCAGGA70.10105384726432798No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG70.10105384726432798No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAAATATTT70.10105384726432798No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA70.10105384726432798No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)