FastQCFastQC Report
Tue 7 May 2024
EGAF00007822180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8052
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTA5156.395926477893692No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5126.358668653750621No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3244.023845007451564No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGTCGCC1962.4341778440139095Illumina Single End PCR Primer 1 (95% over 23bp)
CTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGG1261.564828614008942No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTC740.9190263288623944No Hit
ATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGG550.6830601092896175No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTG420.5216095380029807No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.4222553402881272No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.4098360655737705No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGG320.39741679085941384No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG310.3849975161450572No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGGCGCC300.37257824143070045No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC300.37257824143070045No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTT280.34773969200198707No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC220.273224043715847No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG210.26080476900149036No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA210.26080476900149036No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG180.22354694485842028No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG180.22354694485842028No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC170.2111276701440636No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGGGGTCGCC170.2111276701440636No Hit
CATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGTCGGC170.2111276701440636Illumina Single End PCR Primer 1 (95% over 21bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT150.18628912071535023No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTC150.18628912071535023No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA150.18628912071535023No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC140.17386984600099353No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA130.16145057128663687No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA120.14903129657228018No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTG120.14903129657228018No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGA120.14903129657228018No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.14903129657228018No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA120.14903129657228018No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA110.1366120218579235No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA100.12419274714356682No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.12419274714356682No Hit
ATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATCTCGGTGGTCGCCG90.11177347242921014Illumina RNA PCR Primer (95% over 23bp)
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT90.11177347242921014No Hit
GTCTCTTATACACATCTGACGCTGCCGACGATACTTCCTGGGTGTAGATC90.11177347242921014No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC90.11177347242921014No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)