FastQCFastQC Report
Tue 7 May 2024
EGAF00007822186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13055
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5794.435082343929529No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGTA3542.711604749138261No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3492.6733052470317884No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGGT2031.5549597855227881No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGTT2001.5319800842589046No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGGA1391.0647261585599386No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.7047108387590961No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGG710.5438529299119111No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTG690.5285331290693221No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC510.39065492148602066No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTT470.3600153198008426No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC440.337035618536959No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.32937571811566446No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA400.3063960168517809No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG380.29107621600919187No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG320.24511681348142475No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC300.2297970126388357No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG270.2068173113749521No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG270.2068173113749521No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT250.19149751053236308No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGT240.18383761011106856No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTT240.18383761011106856No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGTG230.17617770968977403No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.16085790884718498No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC210.16085790884718498No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC200.15319800842589046No Hit
CTTCACCGAGTCGTACGAACCAGCAATGCCCATAGTTCCCGAGTCGAGCA200.15319800842589046No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.14553810800459593No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTTGGAGGGTGTA180.1378782075833014No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG180.1378782075833014No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTTGGAGGGTGGA170.13021830716200689No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGT170.13021830716200689No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA160.12255840674071238No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA160.12255840674071238No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA160.12255840674071238No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAA150.11489850631941785No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAA150.11489850631941785No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT140.10723860589812334No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGGG140.10723860589812334No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)