FastQCFastQC Report
Tue 7 May 2024
EGAF00007822191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9994
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCG4374.3726235741444865No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCT3333.331999199519712No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2512.511506904142485No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1661.6609965979587753No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG610.6103662197318391No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCAT590.5903542125275165No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.5803482089253552No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC410.4102461476886132No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT380.38022813688212925No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTG370.37022213327996795No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACC350.3502101260756454No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT340.3402041224734841No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCG300.3001801080648389No Hit
ATTGCGCAATGAACGGTGCGGGCCAGACCGGCCGCACTCTTGCCCCTCCT300.3001801080648389No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG270.27016209725835505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT210.21012607564538724No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC200.20012007204322593No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG200.20012007204322593No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG170.17010206123674204No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCAGCG160.16009605763458074No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT160.16009605763458074No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC160.16009605763458074No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC150.15009005403241946No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCT150.15009005403241946No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT150.15009005403241946No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA150.15009005403241946No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCGCG140.14008405043025815No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT130.13007804682809684No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACC130.13007804682809684No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG130.13007804682809684No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG120.12007204322593557No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATT110.11006603962377427No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT110.11006603962377427No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT110.11006603962377427No Hit
ATTGCGCAATCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA110.11006603962377427No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT110.11006603962377427No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCT110.11006603962377427No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCTCG100.10006003602161297No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCAGCT100.10006003602161297No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC100.10006003602161297No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT100.10006003602161297No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT100.10006003602161297No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)