FastQCFastQC Report
Tue 7 May 2024
EGAF00007822193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8498
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGTATGATCG2452.883031301482702No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2122.494704636385032No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGTATGATCT1782.0946104965874324No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1251.4709343374911743No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA921.0826076723935045No Hit
ATTGCGCAATGATCTACACGGGCTCCGCGAAATGAAAGGGGAGATATGTC470.5530713108966816No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG440.5177688867968934No Hit
ATTGCGCAATGAGGCAAGGGGGGGGGTGACGCAGTGTGGTAGCCAAGCCA360.42362908919745823No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAT330.38832666509767005No Hit
ATTGCGCAATGAGTCGAGGGGGGGGGTGACGCAGTGTGGTAGCCAAGCCA310.36479171569781127No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC310.36479171569781127No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGTAT310.36479171569781127No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGTATGAT300.3530242409978819No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT280.3294892915980231No Hit
ATTGCGCAATGCTTAGGAGGGGCCATCATCTTGGCCAAGCTTTCCATCGT270.3177218168980937No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT230.27065191809837613No Hit
ATTGCGCAATGTCATACCGGGGGATAACTAGCTGTTCGGGCTCATAGAGC230.27065191809837613No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTGATGGTATGATCG220.2588844433984467No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC190.2235820192986585No Hit
ATTGCGCAATGTGAACCGTGGGGGTTCCAGGAAATAGCCCCTCCATTATA180.21181454459872912No Hit
ATTGCGCAATGTTTAGGGCGGGATCCCCTCGACCACTCCTTTGGCGCTTC180.21181454459872912No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT170.20004706989879972No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT170.20004706989879972No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA170.20004706989879972No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC170.20004706989879972No Hit
ATTGCGCAATGATATATAGGGGCATCCGCAGATTGTGGATGCAGCCGTGA150.17651212049894094No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG140.16474464579901155No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG140.16474464579901155No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATG130.15297717109908215No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGTAGGATCG110.12944222169922334No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG110.12944222169922334No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT110.12944222169922334No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG110.12944222169922334No Hit
ATTGCTGACTCTTAATTCCTATTTCAATATGTGTTTCCTTGAAGAATTCA110.12944222169922334No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC110.12944222169922334No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG110.12944222169922334No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT100.11767474699929395No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGTA100.11767474699929395No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC100.11767474699929395No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC100.11767474699929395No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG100.11767474699929395No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGGATGATCG100.11767474699929395No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)