FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8498
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT2893.400800188279595No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGTA1661.9534008001882794No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1651.94163332548835No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGTT1051.2355848434925865No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGGT901.0590727229936456No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGGA840.9884678747940692No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.7060484819957638No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGG370.4353965638973876No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC360.42362908919745823No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTT290.3412567662979525No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTG290.3412567662979525No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.3294892915980231No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG250.2941868674982349No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA230.27065191809837613No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC210.2471169686985173No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG190.2235820192986585No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG190.2235820192986585No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC190.2235820192986585No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG170.20004706989879972No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC160.18827959519887033No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC160.18827959519887033No Hit
GGTCCCCCTTCTCCCGAAGTTACGGGGGAATTTTGCCGAATTCCTTAACC150.17651212049894094No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC150.17651212049894094No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT140.16474464579901155No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA130.15297717109908215No Hit
GGATCACCAACATCACCCAAAAAAGGGCTACGCATCACGCCTCACACACA130.15297717109908215No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT130.15297717109908215No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA120.14120969639915276No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA120.14120969639915276No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA120.14120969639915276No Hit
CATTTTTTTTTCACTACCTTCTGTTTGACGTTCCTTCATCCAAATCTTTT110.12944222169922334No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT110.12944222169922334No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTTGGAGGGTGTA110.12944222169922334No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.12944222169922334No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA100.11767474699929395No Hit
CATTTTTTTTCACTACCTTCTGTTTGACGTTCCTTCATCCAAATCTTTTC100.11767474699929395No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT100.11767474699929395No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATTTAT90.10590727229936456No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA90.10590727229936456No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA90.10590727229936456No Hit
CATCTGACGCTGCCGACGATTGTTGGAGGGTGTAGATCTCGGTGGTCGCC90.10590727229936456Illumina RNA PCR Primer (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTTGGAGGGTGGT90.10590727229936456No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGA90.10590727229936456No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG90.10590727229936456No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.10590727229936456No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.10590727229936456No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA90.10590727229936456No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA90.10590727229936456No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAGGGTGTG90.10590727229936456No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTTGGAG90.10590727229936456No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)