FastQCFastQC Report
Tue 7 May 2024
EGAF00007822195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11254
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCG4013.563177536875777No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4003.554291807357384No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCT2582.292518215745513No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2282.0259463301937086No Hit
ATTGCGCAATGACGATACTGGGATTCAAAGCTTCAGATGATGCAGCACTT1110.986315976541674No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.9241158699129198No Hit
ATTGCGCAATGAAGGGCATGGGGGACCTGTAGCTCAGCTGGTTAGAGCGC920.8174871156921982No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG750.6664297138795094No Hit
ATTGCGCAATGTAGTCTTTGGGAAGGATGTGAGGAGGAAGACGCAGTGAA710.6308867958059357No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC620.5509152301403945No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT590.524258041585214No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCAC540.4798293939932468No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT530.4709436644748534No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAAT530.4709436644748534No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT500.444286475919673No Hit
ATTGCGCAATGCGGAACGGGGGGCTTTTTTTTCTGAGCCCCGGCGGTGCG470.4176292873644926No Hit
ATTGCGCAATGGCTAATATGGGGAATTGAAGAGTTTGATCATGGCTCAGA460.4087435578460991No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT430.38208636929091877No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC340.3021148036253776No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG330.2932290741069842No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTC300.2665718855518038No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG270.2399146969966234No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA270.2399146969966234No Hit
ATTGCGCAATGGGCCACGCGGGGAAGGGCCATCGCTCAACGGATAAAAGG240.21325750844144306No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG230.20437177892304956No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAAGCG220.1954860494046561No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT220.1954860494046561No Hit
ATTGCGCAATGTCGTCACCGGGGGGGTATAGGAATATGAAAAGAGTGGGG210.18660031988626266No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC200.17771459036786919No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT200.17771459036786919No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC190.16882886084947574No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG190.16882886084947574No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT180.15994313133108226No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCGCG170.1510574018126888No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCG170.1510574018126888No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT170.1510574018126888No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC170.1510574018126888No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT170.1510574018126888No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC160.14217167229429536No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCT150.1332859427759019No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG150.1332859427759019No Hit
ATTGCGCAATGTTTGCCAAGGGGGGCCCATAGCTCAGTTGGTAGAGCAAC150.1332859427759019No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTG140.12440021325750845No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCTCG130.11551448373911498No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCA130.11551448373911498No Hit
ATTGCGCAATGCACTACAGGGAAAGGGCGCACGGTGGATGCCTTGGCACT130.11551448373911498No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGC130.11551448373911498No Hit
ATTGCGCAATGTCGTACCGGGGGGGTATAGGAATATGAAAAGAGTGGGGA120.10662875422072153No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT120.10662875422072153No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)