FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8022
Sequences flagged as poor quality0
Sequence length151
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCG3234.026427324856644No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2913.6275243081525805No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCT1972.455746696584393No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1571.957117925704313No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.6731488406881078No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTA450.5609573672400897No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG440.5484916479680878No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGAT440.5484916479680878No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCG350.4363001745200698No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT300.3739715781600598No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC290.36150585888805786No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCG260.3241087010720518No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT250.31164298180004985No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG240.2991772625280479No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA230.28671154325604586No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT220.2742458239840439No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT200.24931438544003987No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG180.22438294689603588No Hit
ATTGCGCAATGCCAAGGTTGGGGAGACGCCTGCACAACAAGGAGCATGTG180.22438294689603588No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC170.21191722762403392No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGAGCG170.21191722762403392No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG170.21191722762403392No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC170.21191722762403392No Hit
ATTGCGCAATGGGTGTCCGGGGCTAAGTGTGTGGACTGTCTGGTAGGTAA160.19945150835203193No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC160.19945150835203193No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG150.1869857890800299No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCT150.1869857890800299No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC140.17452006980802792No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT130.1620543505360259No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTA130.1620543505360259No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC110.13712291199202195No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGAGCT110.13712291199202195No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCGCG110.13712291199202195No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG100.12465719272001993No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC90.11219147344801794No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT90.11219147344801794No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC90.11219147344801794No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCT90.11219147344801794No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT90.11219147344801794No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)