FastQCFastQC Report
Tue 7 May 2024
EGAF00007822199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13498
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCG5393.9931841754333974No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCT4413.2671506889909616No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3302.4448066380204474No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1881.3927989331752852No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC1240.9186546154985924No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1150.8519780708253075No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.7260334864424359No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTAT900.6667654467328493No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT780.5778633871684694No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT690.5111868424951845No Hit
ATTGCGCAATGAATATACTGGGGGGCTGAGCCGCGGCAGGAGAGTACTGA650.4815528226403912No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC640.4741443176766928No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGG600.44451029782189955No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGT590.4371017928582012No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT580.42969328789450284No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA530.39265076307601127No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT460.340791228330123No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC460.340791228330123No Hit
ATTGCGCAATGTGTCCCGGGGGTGCTCCAGGGCAGCGGAACGGGGTGACC390.2889316935842347No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG360.26670617869313973No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC340.2518891687657431No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC340.2518891687657431No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT330.24448066380204475No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG320.2370721588383464No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG310.2296636538746481No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGGTATCG290.21484664394725142No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCG280.20743813898355312No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG280.20743813898355312No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC260.19262112905615647No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCGCG250.18521262409245814No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC240.17780411912875982No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT240.17780411912875982No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT230.1703956141650615No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT210.15557860423766484No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT200.14817009927396652No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGG200.14817009927396652No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG190.1407615943102682No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG180.13335308934656986No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG170.12594458438287154No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC170.12594458438287154No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGTTATCTCG170.12594458438287154No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTCT160.1185360794191732No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGA160.1185360794191732No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGGAGCGGTATCT150.11112757445547489No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT150.11112757445547489No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT140.10371906949177656No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT140.10371906949177656No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCTG140.10371906949177656No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)