FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14826
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC5813.9187913125590184No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCG5203.507351949278295No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCT4873.2847699986510186No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3782.5495750708215295No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1581.0656953999730203No Hit
ATTGCGCAATGTAGCTAAGGGGGGTTAAGCGACTAAGCGTACACGGTGGA1230.8296236341562121No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1080.7284500202347227No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCGATATATCG1070.7217051126399568No Hit
ATTGCGCAATGGGTGATACGGGGGTTAAGCGACTAAGCGTACACGGTGGA950.6407662215027654No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATA890.6002967759341696No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC810.546337515176042No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAC790.5328476999865102No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATAT760.5126129772022123No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCGATATATCT710.47888843922838253No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT670.4519088088493188No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT640.43167408606502095No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT580.3912046404964252No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT500.33724537973829755No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA440.2967759341697019No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG440.2967759341697019No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC430.2900310265749359No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC410.276541211385404No Hit
ATTGCGCAATGTTCTTGGAGGGCATGGATGTGGTACGGAAGGTGGAGAGC410.276541211385404No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG400.26979630379063807No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT370.24956158100634024No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG340.22932685822204235No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCG330.22258195062727643No Hit
ATTGCGCAATGTGCCTTACGGGGAGACGGAGAAGGGGGGGGGAGGAGGAG330.22258195062727643No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC320.21583704303251047No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAC310.2090921354377445No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT310.2090921354377445No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG300.20234722784297854No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA290.1956023202482126No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACA290.1956023202482126No Hit
ATTGCGCAATGAAGGTGCTGGGGTTCAAGGTTTAACATTAAAATTAACTA290.1956023202482126No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC280.18885741265344666No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCT270.18211250505868068No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC260.17536759746391475No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT260.17536759746391475No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC260.17536759746391475No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC250.16862268986914877No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTG240.16187778227438285No Hit
ATTGCGCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACA230.1551328746796169No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG230.1551328746796169No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG230.1551328746796169No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGAT220.14838796708485094No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT220.14838796708485094No Hit
ATTGCGCAATGTCATCCCGGGGATGGGGGAACCCAACATGAGTTATGTCA220.14838796708485094No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCCT210.141643059490085No Hit
ATTGCGCAATGTTGCACGTGGGGGTTAAGCGACTAAGCGTACACGGTGGA210.141643059490085No Hit
ATTGCGCAATGTATTACGAGGGGGTTAAGCGACTAAGCGTACACGGTGGA210.141643059490085No Hit
ATTGCGCAATGAGGGCTGGGGGGAAGCACCGGCTAACTACGTGCCAGCAG200.13489815189531904No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT190.12815324430055308No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGC180.12140833670578711No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT180.12140833670578711No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG170.11466342911102118No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCGCG170.11466342911102118No Hit
ATTGCGCAATGTATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGG160.10791852151625524No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT160.10791852151625524No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT160.10791852151625524No Hit
GGGGGGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160.10791852151625524No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCTCG150.10117361392148927No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT150.10117361392148927No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTCT150.10117361392148927No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTG150.10117361392148927No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)