FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14826
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT7394.984486712532038No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGTA6434.336975583434507No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC4723.183596384729529No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGTT2341.5783083771752329No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGGA1010.6812356670713611No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.546337515176042No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTA790.5328476999865102No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTC740.4991231620126805No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTG730.4923782544179145No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGGT660.44516390125455285No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC590.39794954809119115No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.39794954809119115No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC570.38445973290165925No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA450.3035208417644678No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG440.2967759341697019No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG430.2900310265749359No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG380.25630648860110616No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCGAGTGTA340.22932685822204235No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG320.21583704303251047No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC290.1956023202482126No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTC290.1956023202482126No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTATAGTGTA280.18885741265344666No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA270.18211250505868068No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCT270.18211250505868068No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC270.18211250505868068No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC260.17536759746391475No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA240.16187778227438285No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT230.1551328746796169No Hit
CATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCT230.1551328746796169No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCT210.141643059490085No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC210.141643059490085No Hit
CTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGTAGATCTCGG210.141643059490085No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCT210.141643059490085No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.13489815189531904No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT190.12815324430055308No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA190.12815324430055308No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC190.12815324430055308No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG180.12140833670578711No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA170.11466342911102118No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA170.11466342911102118No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.11466342911102118No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA160.10791852151625524No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTATAGTGTT150.10117361392148927No Hit
ATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTC150.10117361392148927No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC150.10117361392148927No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.10117361392148927No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)