FastQCFastQC Report
Tue 7 May 2024
EGAF00007822208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12367
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5244.237082558421606No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGTA5174.18048031050376No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3162.5551871917198996No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGTT1851.4959165521144984No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.6064526562626344No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTC650.5255923020942832No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGGA610.4932481604269427No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTG560.452817983342767No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC530.4285598770922616No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC510.4123878062585914No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTA500.4043017708417563No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGGT410.33152745209024015No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.33152745209024015No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG350.28301123958922936No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG330.26683916875555913No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC330.26683916875555913No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG310.2506670979218889No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA300.2425810625050538No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC270.21832295625454837No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG270.21832295625454837No Hit
CACAGGTGCACAGAGCTGCTTCTTGGCTGAAGAGTGACAAAAGTCCTCCC260.21023692083771325No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCT240.19406485000404303No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC230.18597881458720789No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.16980674375353763No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTATAGTGTA210.16980674375353763No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA200.16172070833670252No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT200.16172070833670252No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC200.16172070833670252No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCGAGTGTA190.1536346729198674No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA180.14554863750303226No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC180.14554863750303226No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA170.13746260208619712No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA170.13746260208619712No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTC170.13746260208619712No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCT150.1212905312525269No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT150.1212905312525269No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA150.1212905312525269No Hit
CTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGTAGATCTCGG140.11320449583569175No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA130.10511846041885663No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG130.10511846041885663No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.10511846041885663No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.10511846041885663No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)