FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8625
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGTATGATCG3303.826086956521739No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3193.698550724637681No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGTATGATCT2613.0260869565217394No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2302.666666666666667No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.9043478260869564No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG510.591304347826087No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT440.5101449275362319No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT410.4753623188405797No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC370.4289855072463768No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGTATGAT350.4057971014492754No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGTAT340.39420289855072466No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAT330.3826086956521739No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT270.3130434782608696No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT250.2898550724637681No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC240.2782608695652174No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG200.2318840579710145No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC190.2202898550724638No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG180.20869565217391303No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC170.19710144927536233No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA170.19710144927536233No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG170.19710144927536233No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGA150.17391304347826086No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG130.1507246376811594No Hit
ATTGCGCAATGATGCCTGCGGGTACGGACAAGGGGAATCTGACTGTCTAA130.1507246376811594No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT120.1391304347826087No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC120.1391304347826087No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTGATGGTATGATCG120.1391304347826087No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC120.1391304347826087No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120.1391304347826087No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT120.1391304347826087No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT110.12753623188405797No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGTATGATCGCG110.12753623188405797No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT110.12753623188405797No Hit
ATTGCGCAATGAGGGCTGCGGGGAGAAACCAGAGAAGAGACGAAGGTGTA110.12753623188405797No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGTATGAGCG100.11594202898550725No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG100.11594202898550725No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGTAGGATCG100.11594202898550725No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC100.11594202898550725No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT90.10434782608695652No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATG90.10434782608695652No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)