FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8625
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4044.684057971014493No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGTA3854.463768115942028No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2663.084057971014493No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGTT1231.4260869565217391No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.591304347826087No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.4869565217391304No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGGA370.4289855072463768No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC340.39420289855072466No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTA340.39420289855072466No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTG330.3826086956521739No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTC330.3826086956521739No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG280.32463768115942027No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC270.3130434782608696No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG250.2898550724637681No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA250.2898550724637681No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG230.26666666666666666No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGGT220.25507246376811593No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG220.25507246376811593No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCT200.2318840579710145No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC200.2318840579710145No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTATAGTGTA180.20869565217391303No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA170.19710144927536233No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA170.19710144927536233No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT160.1855072463768116No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC150.17391304347826086No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC150.17391304347826086No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG150.17391304347826086No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA140.16231884057971013No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.1507246376811594No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCGAGTGTA130.1507246376811594No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA110.12753623188405797No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA110.12753623188405797No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTATTGTA110.12753623188405797No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTC110.12753623188405797No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC100.11594202898550725No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA100.11594202898550725No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA100.11594202898550725No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAAATGT90.10434782608695652No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA90.10434782608695652No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA90.10434782608695652No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC90.10434782608695652No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)