FastQCFastQC Report
Tue 7 May 2024
EGAF00007822212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9747
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5916.063404124345952No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3833.9294141787216583No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGTA3513.6011080332409975No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGTT1041.0669949728121473No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGGA500.5129783523135324No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTA480.49245921822099104No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.4821996511747204No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC470.4821996511747204No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTG450.46168051708217916No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.3283061454806607No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCTAGTGGT310.31804657843439005No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG290.29752744434184875No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTC290.29752744434184875No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC280.2872678772955781No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC230.2359700420642249No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG230.2359700420642249No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA220.22571047501795424No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT220.22571047501795424No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG220.22571047501795424No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC210.21545090797168356No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA190.1949317738791423No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA180.18467220683287164No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAC180.18467220683287164No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCT170.17441263978660101No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG150.1538935056940597No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA150.1538935056940597No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCT150.1538935056940597No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCT140.14363393864778906No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTCGAGTGTA130.1333743716015184No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.1333743716015184No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTC130.1333743716015184No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA120.12311480455524776No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACTCTCTTATAGTGTA120.12311480455524776No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC120.12311480455524776No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA110.11285523750897712No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA110.11285523750897712No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAT110.11285523750897712No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)