FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9451
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTA4905.184636546397207No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT3613.8197016188763095No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1841.9468839276267063No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGGA600.6348534546608825No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.6136916728388531No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTT460.48672098190667656No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTG420.4443974182626177No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG380.40207385461855893No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.40207385461855893No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTA350.3703311818855148No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG340.35975029097450006No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC330.34916940006348535No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC330.34916940006348535No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG300.31742672733044125No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA300.31742672733044125No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAG280.2962649455084118No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC250.26452227277536766No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG230.24336049095333828No Hit
CTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTAGATCTCGG210.22219870913130885No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGGT200.21161781822029413No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGT190.20103692730927947No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA160.16929425457623534No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT160.16929425457623534No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA160.16929425457623534No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA140.1481324727542059No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC140.1481324727542059No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTACGGA140.1481324727542059No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTA130.1375515818431912No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA130.1375515818431912No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT130.1375515818431912No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC130.1375515818431912No Hit
CTCTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTAGATCTC130.1375515818431912No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.1375515818431912No Hit
CATCTGACGCTGCCGACGATAGTACGGAGGTGTAGATCTCGGTGGTCGCC120.12697069093217647Illumina Single End PCR Primer 1 (95% over 22bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA120.12697069093217647No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA120.12697069093217647No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAT110.11638980002116178No Hit
CTTATACACATCTGACGCTGCCGACGATAGTACGGAGGTGTAGTTCTCGG110.11638980002116178No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA110.11638980002116178No Hit
GGATTTCACAAGAGACTTGATAATCCGCCTACGCGTCCTTTACGCCCAGT110.11638980002116178No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC110.11638980002116178No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGA100.10580890911014706No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA100.10580890911014706No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA100.10580890911014706No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA100.10580890911014706No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGTA100.10580890911014706No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAGTAC100.10580890911014706No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.10580890911014706No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)