FastQCFastQC Report
Tue 7 May 2024
EGAF00007822217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13567
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCG7145.262769956512125No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCT4973.663300656003538No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3772.778801503648559No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2461.8132232623277071No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1350.9950615463993513No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAAT1060.7813075845802314No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTC900.6633743642662343No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG650.4791037075256136No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT630.464362054986364No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAG620.45699122871673914No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC570.42013709736861504No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCG540.39802461855974053No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT480.3537996609419916No Hit
ATTGCGCAATGACTCTAGGGGGGGGGCTTGGGTTCCGGCGACATGGCTAA440.3243163558634923No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC410.3022038770546178No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT400.294833050784993No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT350.25797891943686885No Hit
ATTGCGCAATGGACTGTGGGGGATAAGCTTCATGGTCGAGAGGGAAACAG330.2432372668976192No Hit
ATTGCGCAATGCATCATCTGGGACCTCACGGCCCATGATTACGTCATCCA320.23586644062799442No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG320.23586644062799442No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG320.23586644062799442No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA290.2137539618191199No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG290.2137539618191199No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGT280.2063831355494951No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC270.19901230927987026No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAG250.18427065674062063No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCT240.1768998304709958No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC240.1768998304709958No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA220.16215817793174614No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT210.15478735166212132No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT200.1474165253924965No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGC200.1474165253924965No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG200.1474165253924965No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT190.14004569912287168No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAAGCG190.14004569912287168No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC190.14004569912287168No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC180.13267487285324686No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCTCG180.13267487285324686No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATGG180.13267487285324686No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCCATCG170.12530404658362201No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAAGCT170.12530404658362201No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT160.11793322031399721No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG160.11793322031399721No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG160.11793322031399721No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGTC140.10319156777474756No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG140.10319156777474756No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG140.10319156777474756No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)