FastQCFastQC Report
Tue 7 May 2024
EGAF00007822227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10956
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCG5545.056589996349032No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC4113.751369112814896No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCT3343.048557867834976No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2652.41876597298284No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA880.8032128514056224No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT650.5932822197882439No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAAT590.538517707192406No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG580.529390288426433No Hit
ATTGCGCAATGTTTTGCCCGGGGAATTGCTGCCAGTGGCCACTTCATCTG570.52026286966046No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT540.49288061336254113No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCAC510.4654983570646221No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC510.4654983570646221No Hit
ATTGCGCAATGTAGTGCTGGGGGGCCTAGTGGAGTGAGGGGTAACAAGAT460.4198612632347572No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCG420.3833515881708653No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT410.3742241694048923No Hit
ATTGCGCAATGGCCATACCGGGCGATGAAGGACGTGATAGCCTGCGAAAA370.33771449434100037No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT360.32858707557502737No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTC350.31945965680905436No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG290.2646951442132165No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC270.24644030668127057No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT260.23731288791529756No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC250.22818546914932458No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCA250.22818546914932458No Hit
ATTGCGCAATGCACACGGGGGGTCCCGCAACGAGCGCAACCCTTGTCCTT250.22818546914932458No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA240.21905805038335158No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC230.2099306316173786No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG230.2099306316173786No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT220.2008032128514056No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG220.2008032128514056No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAAGCG210.19167579408543264No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC170.1551661190215407No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT170.1551661190215407No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG160.14603870025556773No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT160.14603870025556773No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC160.14603870025556773No Hit
ATTGCGCAATGCGGCGGCGGGGGAAGCAGCGTGAGCAGCCGGAGGATCGC150.13691128148959475No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCT150.13691128148959475No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC150.13691128148959475No Hit
ATTGCGCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTC150.13691128148959475No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATGG140.12778386272362174No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT130.11865644395764878No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG130.11865644395764878No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAAGCT130.11865644395764878No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG130.11865644395764878No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC120.10952902519167579No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC120.10952902519167579No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT120.10952902519167579No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCGCG110.1004016064257028No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT110.1004016064257028No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCTCG110.1004016064257028No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT110.1004016064257028No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG110.1004016064257028No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)