FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2374
Sequences flagged as poor quality0
Sequence length151
%GC64

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT25410.699241786015163No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC1546.486941870261162No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGGGGGGGGGGGGG251.0530749789385003No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC210.8845829823083403No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.7160909856781803No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGGGGGGGGGGGGG170.7160909856781803No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGTAGATCTCGGTGGTCGCC160.6739679865206403Illumina Single End PCR Primer 1 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGT130.5475989890480202No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGG110.4633529907329402No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGTGGGGGGGGGGG110.4633529907329402No Hit
ATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGGGGGGGG100.42122999157540014No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGTGGG100.42122999157540014No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.37910699241786017No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG80.33698399326032014No Hit
CTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGGG70.2948609941027801No Hit
CTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGTG70.2948609941027801No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG60.2527379949452401No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGGCGGGGGGGGGG60.2527379949452401No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC60.2527379949452401No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA50.21061499578770007No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCT50.21061499578770007No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGTGTGGTGGGGGGGGGGG50.21061499578770007No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG50.21061499578770007No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGGGGG50.21061499578770007No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGTTGGGGG50.21061499578770007No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGTGGGGGG50.21061499578770007No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA50.21061499578770007No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGTGGGGGGGG50.21061499578770007No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGTGGGGGGGGGGGGGG50.21061499578770007No Hit
CTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGGG50.21061499578770007No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAG50.21061499578770007No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGTGTTGTGGGGGGGGGGG40.16849199663016007No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGGCGGGGGGGGGG40.16849199663016007No Hit
ATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGTGGGGGG40.16849199663016007No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.16849199663016007No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGTGGG40.16849199663016007No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGTGGTGGGGGGGGGGG40.16849199663016007No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC40.16849199663016007No Hit
ATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCG40.16849199663016007No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGAAAA40.16849199663016007No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA40.16849199663016007No Hit
ATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGTGGTGGGGGGGG40.16849199663016007No Hit
CTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGTG30.12636899747262004No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT30.12636899747262004No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATG30.12636899747262004No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGTTGTGGGGGGGGGGG30.12636899747262004No Hit
CTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGTGGTGTG30.12636899747262004No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGTGGGTGGGGGGG30.12636899747262004No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTG30.12636899747262004No Hit
GTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12636899747262004No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC30.12636899747262004No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGGGGG30.12636899747262004No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGTGGGGGGGGGGG30.12636899747262004No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAA30.12636899747262004No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA30.12636899747262004No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGTGTTGTGGG30.12636899747262004No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30.12636899747262004No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.12636899747262004No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGTGGTGGGGGGGGTGGGG30.12636899747262004No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGTGTGGGGGGGGGGGGGG30.12636899747262004No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.12636899747262004No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA30.12636899747262004No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGTTGTGGGTGGTGGGG30.12636899747262004No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGTATATCTCGGTGGTCGCC30.12636899747262004Illumina RNA PCR Primer (95% over 21bp)
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGTGGGGTGGGGGGGGGGG30.12636899747262004No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)