FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13103
Sequences flagged as poor quality0
Sequence length151
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCG6224.747004502785622No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCT3722.8390444936274135No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3472.6482484927115926No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2461.8774326490116768No Hit
ATTGCGCAATGAAATGTTCGGGGAGAAAGCGCCGAGAGAAGCAAGATGGC1110.8471342440662444No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCG1020.7784476837365489No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020.7784476837365489No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAAT740.5647561627108295No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTC720.5494924826375639No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG710.541860642600931No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAG620.4731740822712356No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT500.3815920018316416No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATCT410.3129054415019461No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT410.3129054415019461No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT400.3052736014653133No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG340.2594825612455163No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC300.22895520109898496No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA290.22132336106235212No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAATGG280.21369152102571928No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG270.20605968098908645No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGC270.20605968098908645No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG260.1984278409524536No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCCA250.1907960009158208No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG250.1907960009158208No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC220.1679004808059223No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAAGCG210.16026864076928948No Hit
ATTGCGCAATGGAGGCGGGGGGAAGATGGCGGAGTCCGGCGGTAGCAGCG190.1450049606960238No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC180.13737312065939097No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC160.1221094405861253No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT160.1221094405861253No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC160.1221094405861253No Hit
ATTGCGCAATGGTTTTGGTGGGGAGTGTGTAAGAGCGCATGGTGGATGCC160.1221094405861253No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAGCTGTCAAGCT160.1221094405861253No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT140.10684576051285964No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT140.10684576051285964No Hit
ATTGCGCAATGCCCGTATAGGGGCCTTTTCCGGTCGCGGCGCTGCGCGGT140.10684576051285964No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)