FastQCFastQC Report
Tue 17 Jan 2023
EGAF00007822236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10256
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4684.563182527301092No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGG3333.246879875195008No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3133.0518720748829953No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGT3032.954368174726989No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGTT770.750780031201248No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGTA750.7312792511700468No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAG650.6337753510140406No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTG630.6142745709828393No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGTG580.5655226209048362No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGA500.4875195007800312No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC450.43876755070202805No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCT420.4095163806552262No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC350.34126365054602187No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.3217628705148206No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.31201248049922No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG320.31201248049922No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG300.2925117004680187No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA290.2827613104524181No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCT250.2437597503900156No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG250.2437597503900156No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC250.2437597503900156No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG220.21450858034321374No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT220.21450858034321374No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGA210.2047581903276131No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGTGGG200.19500780031201248No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGC190.18525741029641185No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA180.17550702028081125No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGGGGGGGGGGGGG180.17550702028081125No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC180.17550702028081125No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT170.16575663026521062No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC160.15600624024961No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGGGGGGGGGGGGG160.15600624024961No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGGGGG150.14625585023400936No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC150.14625585023400936No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA140.13650546021840873No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTA140.13650546021840873No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.13650546021840873No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA130.12675507020280813No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA130.12675507020280813No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAA130.12675507020280813No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA130.12675507020280813No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGGGGG120.1170046801872075No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGTAGATCTCGGTGGTCGCC120.1170046801872075Illumina Single End PCR Primer 1 (95% over 22bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAA120.1170046801872075No Hit
CATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTA110.10725429017160687No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA110.10725429017160687No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA110.10725429017160687No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATGTA110.10725429017160687No Hit
CTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGTG110.10725429017160687No Hit
CTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGGG110.10725429017160687No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)