FastQCFastQC Report
Tue 7 May 2024
EGAF00007822243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8653
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3744.322200392927308No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCG2432.808274586848492No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2292.6464809892522827No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCT1571.8143996301860625No Hit
ATTGCGCAATGCGCCAAACGGGGTGGTCAGGTTGAAGATACTAAGGCGTT850.9823182711198428No Hit
ATTGCGCAATGTAAGACGAGGGCGAAGCGGTGTGATCTATCCATGGCCAG830.9592049000346701No Hit
ATTGCGCAATGGCCTGGATGGGGACATTGAGCGCCGACACCCCGAGACGG830.9592049000346701No Hit
ATTGCGCAATGTCTAGGTCGGGGTGCTGCGGCGAGCGAGCTCATACTAAC680.7858546168958742No Hit
ATTGCGCAATGTAGACTCCGGGGCTCTTTCCGGTGTGGAGTCTGGAGACG620.716514503640356No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.6125043337570785No Hit
ATTGCGCAATGCACGCCAATGGGCGGGACCTAAGGCGAGGCCGACAGGCG480.5547209060441465No Hit
ATTGCGCAATGAAAAGGGAGGGGTGACGGGTGGGAATCCCGTCCGCCGAT430.49693747833121465No Hit
ATTGCGCAATGAGTCGGGTGGGGTGCCGCTCTTGCGGCAGGCGCCGATTG420.4853807927886282No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCG400.46226742170345547No Hit
ATTGCGCAATGCATTTCCCGGGGTACGCTGAAAGCGTCGGGCTGTACACA390.4507107361608691No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCAC370.4275973650756963No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG350.4044839939905235No Hit
ATTGCGCAATGATATACGGGGGGAGAACCTTGAAGACTGAAGTGGAGAAA340.3929273084479371No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAAT330.38137062290535073No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC300.34670056627759155No Hit
ATTGCGCAATGGCAGTGGGGGGGCCGAAATGTGAATTATACTAGAATCAA290.3351438807350052No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT290.3351438807350052No Hit
ATTGCGCAATGTATCACACGGGGGCCGCCGCAGGCACGACGCGCGAGGCC290.3351438807350052No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT280.32358719519241885No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG270.3120305096498324No Hit
ATTGCGCAATGATCACCTCGGGGGGTATATCGGGCCCTGGCGGGAAGCCG260.30047382410724605No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT250.28891713856465967No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT250.28891713856465967No Hit
ATTGCGCAATGGAGATTCTGGGGGGTTCAGAACGTCGTGAGACAGTTCGG230.26580376747948686No Hit
ATTGCGCAATGTTACGATCGGGGAAGCGCGTGACCGTCGCCAAGATGGAT220.25424708193690054No Hit
ATTGCGCAATGGCTGTGTGGGACAGGCGAAGTAGTCACACCACCAGTGGA200.23113371085172774No Hit
ATTGCGCAATGCTATGGACGGGCTTCCTTGAGTCGGATGACACGACGTTC190.21957702530914133No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC180.20802033976655496No Hit
ATTGCGCAATGGTTATAACGGGGTTATTAAGGGCGCATGGTGGATGCCTT180.20802033976655496No Hit
ATTGCGCAATGACTCGGCGGGGGTCCGCAGGAAGTGGGTGCGTTGCAGCA170.19646365422396855No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG170.19646365422396855No Hit
ATTGCGCAATGGGGATCGGGGGGGTCTCTATCCGCCGCGCGCGTTGAAAC170.19646365422396855No Hit
ATTGCGCAATGTAGGCCTGGGGGTCGCGTTAAGCGCCGTCGCATGAAGTC170.19646365422396855No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTC160.18490696868138218No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC160.18490696868138218No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG150.17335028313879577No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT150.17335028313879577No Hit
ATTGCGCAATGATTGACCAGGGAGGATACAAGTCGATCGGTGACTGGCAC150.17335028313879577No Hit
ATTGCGCAATGGGGAGCGATGGGCTGTTTGATAATCTGTATGACGAGGAC130.15023691205362302No Hit
ATTGCGCAATGGGGATCCTGGGGCGACATAAATCCGTCGAGCAAAGAGTA130.15023691205362302No Hit
ATTGCGCAATGTGGTGAACGGGAAGGGCGCATGGTGGATGCCTTGGCATG130.15023691205362302No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC120.13868022651103662No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA120.13868022651103662No Hit
ATTGCGCAATGGCTTTTGTGGGGTTGTGGGTGAGTGAGTGATCCTGTACT120.13868022651103662No Hit
ATTGCGCAATGCTTAGACCGGGGGTGCGAGCAGTACCGAGTCCGCGGCCG110.12712354096845027No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT110.12712354096845027No Hit
ATTGCGCAATGGGGAGCTAGGGGCTGTTTGATAATCTGTATGACGAGGAC110.12712354096845027No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG110.12712354096845027No Hit
ATTGCGCAATGGGACCTCCGGGACCCGTCAAGCCTAAATACTCCTTGGTG100.11556685542586387No Hit
ATTGCGCAATGGAGTCGAGGGGATGCTCGATGTGAACACGGTTGGTGTCT100.11556685542586387No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACACTCG100.11556685542586387No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC100.11556685542586387No Hit
ATTGCGCAATGTCTTAGGTGGGAGGTTTATCGTTTGCGAGAGTTCACATT100.11556685542586387No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC100.11556685542586387No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC100.11556685542586387No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG90.10401016988327748No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC90.10401016988327748No Hit
ATTGCGCAATGAGCGGTTGGGGGTGAGCGAGGTGGCGGGTCCGCCCGTAC90.10401016988327748No Hit
ATTGCGCAATGCTCCCGGGGGGGTGGGTGAGTGAGTGATCCTGTACTGTC90.10401016988327748No Hit
ATTGCGCAATGTAAACCCAGGGGAACGGTTCGCAGTTCTTCATCACGACT90.10401016988327748No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT90.10401016988327748No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)