FastQCFastQC Report
Tue 7 May 2024
EGAF00007822244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8653
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4795.535652374898879No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC2713.131861782040911No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGT1872.161100196463654No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGG1852.1379868253784817No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGTG530.6125043337570785No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.5778342771293193No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGA450.5200508494163874No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGTT440.5084941638738011No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAG390.4507107361608691No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC390.4507107361608691No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGTA370.4275973650756963No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTG340.3929273084479371No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.34670056627759155No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCT280.32358719519241885No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG240.27736045302207324No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGGGGGGGGGGGGG230.26580376747948686No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC210.2426903963943141No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC200.23113371085172774No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG180.20802033976655496No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGTGGGGGGGGGGG170.19646365422396855No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA170.19646365422396855No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG160.18490696868138218No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG140.16179359759620943No Hit
CATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGGGGGGGGGGGGG140.16179359759620943No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.15023691205362302No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA120.13868022651103662No Hit
CTTATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGTGTGGG120.13868022651103662No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA120.13868022651103662No Hit
ATACACATCTGACGCTGCCGACGATGGCTAGGAGGTGGGGGGGGGGGGGG100.11556685542586387No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT100.11556685542586387No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCT100.11556685542586387No Hit
GCCGTCCGAATGAGTACGGTTTCGTTCTGGTGCGTCGGGAACAACGCGTC100.11556685542586387No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTA90.10401016988327748No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGCTAGGA90.10401016988327748No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA90.10401016988327748No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGGC90.10401016988327748No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATATGTA90.10401016988327748No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA90.10401016988327748No Hit
GGTTACTAACCGGTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGTCC90.10401016988327748No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC90.10401016988327748No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)