FastQCFastQC Report
Mon 16 Jan 2023
EGAF00007822253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4400
Sequences flagged as poor quality0
Sequence length151
%GC62

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC50311.431818181818182No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2355.340909090909091No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG521.1818181818181819No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.5681818181818182No Hit
ATTGCGCAATGTGGGCGTGGGGCAAAGCCTGAGGAGGTTACTAACGCTGT190.4318181818181818No Hit
ATTGCGCAATATGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC90.20454545454545456No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCAC90.20454545454545456No Hit
ATTGCGCAATCTGTCTCTTATACACCGGTCTCTTATACACATCTCCGAGC70.1590909090909091No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTCTATCT70.1590909090909091No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACTGTTC60.13636363636363638No Hit
GGGGGGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.13636363636363638No Hit
GGGCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.13636363636363638No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.13636363636363638No Hit
ATTGCGCAATGAAGGACCGGGGGAGAATCGCGCAGGCGCAATTGTGCCCT60.13636363636363638No Hit
ATTGCGCAAACTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC50.11363636363636363No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC50.11363636363636363No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)